Luciferase

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Luciferase

• The enzyme to drive the bioluminescence

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Introduction to Luciferase

• Luciferase, also be called as luminase, is a
  generic terms for enzymes that catalyze the
  process of bioluminescence in nature.
• Luciferin ATP
  Oxyluciferin AMP Light
• The research of luciferase began at the fifth
  ties in twentieth century. The first luciferase
  to be cloned and also the first to be
  structurally characterized is bacterial
  luciferase.

Luciferase
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Classification

• Bacterial Luciferase
• http//filebox.vt.edu/users/chagedor/biol_4684/Met
  hods/bacteria.gif
• Firefly Luciferase
• http//www.mrnussbaum.com/firefly.gif
• Other organism
• http//www.flmnh.ufl.edu/fish/education/adapt/biol
  um.JPG

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Catalytic mechanism of Luciferase
Step 1 Luc LH2 ATP Mg2 ? Luc? LH2
AMP PPi
Luc
Luc? LH2 AMP
Luc
Step 2 Luc? LH2 AMP O2 ? Luc?
Oxyluciferin (EES) AMP CO2
O2
Step 3 Luc? Oxyluciferin (EES) ? Luc?
Oxyluciferin green light
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Step 1 the formation of Luc? LH2 AMP
1. The formation of Luc-AMP complex
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Step 1. The formation of Luc? LH2 AMP
2. The formation of Luc? LH2 AMP active
intermediate
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Step 2 The formation of Luc? Oxyluciferin (EES)
and the leaving of AMP
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Refer1
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Referrence

• 1. Toru Nakatsu, Susumu Ichiyama, Jun Hiratake,
  Adrian Saldanha, Nobuyuki Kobashi, Kanzo Sakata
  and Hiroaki Kato. Structural basis for the
  spectral difference in luciferase
  bioluminescence. Nature 440, 372-376