Erytrocyte Sed' Rate

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Erytrocyte Sed. Rate
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• ???? ??????
• ????? ???? ???? RBC ???? ?? ?????? ?? ?????
  ????? ?? ????
• ?????
• 1- lag phase ? ???? ?? - ????
• 2- ? Decanatation phase ???? ?? ???? ????
• 3- ? Packing phase ???? ???? ??
• ????? ????
• ??????? ??? ?????? ? ???? ??????? ??? ? ESR
• ??????? .????? ????? ??? ? ESR -
  ???????? ESR
• ????? ? ??? ????? ???? ? ???? ? ESR
• ???????? .????????? .???? ???? ? ESR

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Supplies

• Pipettes glass or transparent plastic tube 30 cm
  length not less than2.55 mm
• Pipette rack
• Mechanical suction device
• Diluent
• trisodium citrate 32g/l 109mmol/l Na3Ca6H5O7.
  2H2O

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Time Tem. Of Spec. storage

• Test in diluted sample? must done until 4 hours
  at room Tem
• standing at room tem. RBC ?spheroctye ?ESR
• EDTA Blood ?within 12 hours at 4 degree

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• Ref. Method
• EDTA undiluted blood ?Hctlt0.35
• PCV adjustment 3.5( hct/0.35)-3.5 ml plasma
  require
• Inverted at least 12 time,with air bobble,
  aspirate to
• the ,0,mark of pipette Vertical position,at 18-25
  c,
• Free of vibration sunlight
• Read at 601 min (not include any buffy coat)
• Corrected ESR(undiluted ESR0.86)-12
• Working Method
• EDTA diluted with N. saline(8.5g/l) or
  citrated blood

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• -Quality control
• The reference method can also be used as a
  Quality control procedure for routines tests.
• - EDTA undiluted blood specimen of PCV 0.35
  has ESR (range15-105mm)
• after 60 min.ESR Both Ref. working method
  are read
• -Stabilized whole blood preparation (automated
  system)
• Error
• 1-anticoagulant
• - Higher a mount??ESR
• -Heparin change zeta pot.
• - Difficulty with dilution step

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• 2-vibration, Tilted tube??ESR
• 3-temperature ,?Tem. ??ESR
• 4- Length of tube tube must long enough that
  packing of cells doesnot start before test
  completed
• 5-Verticality
• 4-plastic tube certain plastics attract
  erytrocyte
• 5- bore of tube

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• Automated Semiautomated Systems
• ?Closed system
• -end point is read after 20 min with the tube
  held at an angle of 18 degree

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The end