Growth of phage materials

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Growth of phage materials

• Mod3 Day1
• Tuesday Nov 4, 2008

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BioMaterials Lets start with the biology
1010th phage/ml of seawater
900 nm
2700 copies
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BioMaterials Lets start with the biology
900 nm
2700 copies
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BioMaterials Lets start with the biology

• Infection
• Amplification
• Morphogenesis release of progeny phage
• new M13 phage particles within 10 post-infection
• 1000 phage/cell within the first hour

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BioMaterials Lets start with the biology
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Parts 2 3 of your experiment today

• Phage purification
• Harvest phage infected cells. Phage in
  SUPERNATANT!
• Concentrate by precipitation (PEG/salt)
• Pellet may not be visiblehave faith
• Plaque assay
• Serially dilute phage in water
• Mix with bacteria
• Add warm top agar and plate on LB--gt 37
  overnight
• Give remaining phage to Luis

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BioMaterials now for materials
Oxidized (purple) Reduced (clear)
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Part 1 of your experiment today
Hydrolyze Iridium with citrate at 90 4
hrs Generates Ir colloidal particles Next time
will oxidize to form IrOx colloidal particles
which can react with phage p8 You must wear
safety glasses, gloves, lab coat to do this part
of todays experiment
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FNT

• Describe display technology
• Choose research proposal (5 possible topics)
• or collaborative art/science option