Oxidation%20and%20Reduction%20of%20Hemoglobin

1
Oxidation and Reduction of Hemoglobin
CHEM-342 Introduction to Biochemistry
2
Reversible Reduction of Oxyhemoglobin
Add a small amount of sodium dithionite, Na2S2O4
Stir in the presence of air
3
Spectra for Oxy- and Deoxy-Hemoglobin
OxyHb
DeoxyHb
Wavelength (nm)
OxyHb
DeoxyHb
4
Oxidation of Oxyhemoglobin to Methemoglobin
Add a small amount of Potassium ferricyanide,
K3Fe(CN)6
OxyHb
MetHb
5
Spectra for Carbonmonoxy- and Met-Hemoglobin
CO-Hb
Met-Hb
Wavelength (nm)
Met-Hb
6
Redox Reactions of Hemoglobin
-O2
-O2
O2
-e-
OxyHb
Oxidizing agents -e-
Reducing agents e-
DeoxyHb
MetHb