DNA amplification

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DNA amplification
PCR Polymerase Chain Reaction
Who is Kary Mullis?
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How PCR works
Strand separation (heat 95C)
Cool to 50C so that primers anneal. Each primer
hybridizes to a strand of DNA!
DNA synthesis on both strands 72C (this is the
temp Taq polymerase works best!) Taq- Thermus
aquaticus source of heat stable DNA
polymerase Elongation 5-3 direction (its
polymerase)
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This process is repeated over over

• One tube contains all of the reagents and it is
  thermocycled ( 95 50 72 ) for 30 rounds
  (billion-fold amplification). 1hour

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In what ways is PCR used ?

• Detect viruses (e.g. HIV before immune repsonse)
• Detect bacterial infections
• Monitor cancer therapy
• Forensics
• Obtain gene from genome