MBC 226222224241

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MBC 226-222-224-241

• Name Dr Ayyub Patel
• Offices Biochemistry Computer Rm
• Tel [phone] (txt b4 calling)
• Email ayyub_at_kku.edu.sa
• Web http//medicalcollege.kku.edu.sa

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How to be successful in MBC

• come to class!!!
• read ahead in the text (Harper txtbook 25 or 26th
  edition
• dont fall behind
• understand the material
• memorize structures and pathways
• study in groups
• see me for help during office hours

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College of Medicine Medical Sciences
Introduction Biochemistry?

• What is Biochemistry
• The Aim and Objective of Biochemistry
• Why Biochemistry
• Biomolecules Biochemical Methods
• Summary

? Harper 25th - Edition Pages 1-14
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College of Medicine Medical Sciences
What is Biochemistry

• Bio - Life
• Chemistry - Science concerned with molecules
  and their reactions
• Biochemistry is the chemistry of Life

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College of Medicine Medical Sciences
Aim of Biochemistry

• To Understand all the chemical processes
  associated with living cells
• To Isolate the various molecules and determine
  their structure and function
• To Understand how life began

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College of Medicine Medical Sciences
Why Biochemistry For Medical Students

• All Diseases are a result of abnormalities of
  molecules, chemical reactions and processes
• Medical Professionals should know the normal
  situation so that abnormalities can be quickly
  diagnosed, prognosis of the symptoms can be
  drafted leading to successful treatment of the
  illness.
• Major advances in biochemistry and molecular
  biology has allowed diagnosis and treatment of
  many diseases. Eg. Gene therapy

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Principle Areas of Biochemistry

• Structure and function of biological
  macromolecules
• Metabolism anabolic and catabolic processes.
• Molecular Genetics How life is replicated.
  Regulation of protein synthesis

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College of Medicine Medical Sciences
Biomolecules

• Body is composed of a few elements that combine
  to form many molecules (C, H, N, O, Ca, P, K, Na,
  S, Cl, Mg, Fe, Mn, I)
• Major complex molecules (Proteins, Polysacharides
  (carbohydrates), Lipids, DNA, RNA)
• The Human body has the following major
  constituents
• Proteins (17), Fat (14), Carbohydrates (2),
  Water (62), Minerals (6). ((w/w)

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Organization of Life

• elements
• simple organic compounds (monomers)
• macromolecules (polymers)
• supramolecular structures
• organelles
• cells
• tissues
• organisms

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Range of the sizes of objects studies by
Biochemist and Biologist
1 angstrom 0.1 nm
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Elements of Life
Most abundant, essential for all organisms C, N,
O, P, S, H Less abundant, essential for all
organisms Na, Mg, K, Ca, Cl Trace levels,
essential for all organism Mn, Fe, Co, Cu,
Zn Trace levels, essential for some organisms V,
Cr, Mo, B, Al, Ga, Sn, Si, As, Se, I,
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Important compounds, functional groups
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Many Important Biomolecules are Polymers
lipids
proteins
carbo
nucleic acids
monomer
polymer
supramolecular structure
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Lipids
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Proteins
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Nucleic Acids
monomer
polymer
supramolecular structure
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Prokaryote Cell
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Cellular Organization of an E. coli Cell
200 300 mg protein / mL cytoplasm
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Eukaryote Cell
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College of Medicine Medical Sciences
The Cell

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College of Medicine Medical Sciences
Biochemical Methods

• 3 Major Processes
• (1) Isolation
• (2) Determination of Structure
• (3) Analysis

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College of Medicine Medical Sciences
Biochemical Methods
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College of Medicine Medical Sciences
Biochemical Methods
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College of Medicine Medical Sciences
Summary

• Elements such as C, H, N, O, etc. combine to
  form biomolecules such as proteins, lipids,
  carbohydrates, DNA and RNA.
• Isolation and analysis using various techniques
  allow diagnosis of malfunction and subsequently
  proper treatment of diseases