Standard 96well

1
Standard 96-well plate PCR
SprintTM PCR
Thaw reagents 10 min
Remove foil from plate

• Prepare master mix 15 min
• Enzyme
• 10X Buffer
• dNTPs
• Primers
• water

Dilute primers in water and add mixture 5 min
Add template 5 min
PCR
Aliquot mix 10 min
Add template 5 min
PCR
Total Time
40 min
10 min