4 modern families '''

1
4 modern families ...
Globigerinidae ( 20 sp)
Hastigerinidae (2 sp)
Candeinidae (10 sp)
Globorotaliidae (14 sp)
2
Orbulina universa
3
.. 98 pelagic stations ..
AMT-8
Revelle-2001
OISO-4
AMT-5
4
SSU rDNA
5
Type I
SSU rDNA tree
Type III
Type II
6
Genotypes distribution
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Multiplex PCR approach genetic characterization
of 764 Orbulina universa out of 79 pelagic
stations
8
III
II
I
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Single Cell PCR amplification (ITS rDNA) of 40
Globorotalia truncatulinoides collected along an
Indian Ocean transect. Success rate 90
After PCR
9 genetic diff. with types IV
After RFLP (Sau 96 I)
(20 genetic diff. with types III and IV)
Type I
Type III
Type IV
DNA extraction 4 cents
0.5/reaction !!
PCR amplification 30 cents
RPLF 20 cents
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Atlantic
Sept.-Oct. 1997
Number of individual cells DNA extractions
Jan. 2000
Indian
Absence
1 - 10
10 - 20
20 - 30
gt 30
Dec. 2001
Pacific
13
South- America
Africa
Antarctica
Australia
14
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