Test for immune status

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Test for immune status

• (Innate Immunity
• And
• Humoral mediated immunity)
• Sichon Songsiri

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???????????? immune status

• ??????????????????????????????????????????????????
  ??????
• ??????????????????
• ????????????????????????????????????????????

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??????????????????????

• Innate Immunity
• Adaptive Immunity(HMICMI)

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?????????????????????????????????

• CBC - Neutrophil ,Lymphocyte
• total protein, globulin

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?????????? Innate immunity

• ??????????????????? complement
• ???????????????? phagocytosis
• ??????????????? NK-cells

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???????????????? complement

• ?????????????? nephelometry
• ?????????????? CH50

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????????? complement ?????????????????
nephelometry

• Complement component ???????????????? C3 ,C4
• Principle of test
• C3- Antiserum fresh test serum
• -gt???????????????????????????????????????
• ???????????????? Std.

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?????????? C3, C4

• C3 0.9-1.8 g/l
• C4 0.1-0.4 g/l

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??????? Nephelometer
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????????? complement ?????????? Complement
Hemolysis 50 test

• ???????????????????????? complement ???
  components
• Principle of test
• Sensitized SRBCcomplement
• -gtLYSIS

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????????? CH50

• ?????? sensitized SRBC
• SRBCanti-SRBCGVB ?????????? 5x108 cell/ml
• ????????????? 151 (0.25 ml serum12.5 GVB)

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????????????????????? GVB buffer

• Gelatin
• NaCl
• Na-5,5 diethyl barbiturate
• MgCl2
• CaCl2

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????? ?? CH50
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??? graph ch50
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Assay for NK cell activity

• PBMCK562(labeled with 51Cr
• -gt incubate 4 hr, 37 C, 5CO2
• -gtcentrifuge-gtsupernatant
• -gtgamma count

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??????????? HMI

• ?????? product
• total protein, globulin, electrophoresis,
  immunoelectrophoresis
• ?????? B-cell
• EAC-rosette, Hemolytic plaque assay, ELISPOT

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??????????? HMI

• ?? products
• ??????? B-cell
• ???????? B-cell ???????? specific Ab

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?????? Products ??? HMI

• Total protein
• Globulin
• Gamma-globulin(EP)
• Ig subclass (IEP)

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??????? Nephelometer
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??????? B-cell

• EAC rosette
• FACS
• Immunomagnetic bead
• ???????? B-cell ???????? specific Ab

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??????? B-cell ???????? specific Ab

• Hemolytic Plaque assay
• ELISPOT

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ELISPOT
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?????????? 5 ??????????????????????????????????
??????????????????????????????????????????????????
????????????????????????? innate ??? HMI
?????????????? ??????????????????????
????????????????????????
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Ref.

• http//info.med.yale.edu/labmed/clinical/molecular
  .html
• http//hvelink.saint-lukes.org/library/healthguide
  /MedicalTests/
• http//www.nlm.nih.gov/medlineplus/laboratorytests
  .html
• http//aactg.s-3.com/pub/download/imm/imnk.pdf
• http//path.upmc.edu/divisions/hematopath/fl-cyto3
  .html
• http//www.merck.com/pubs/mmanual_home/contents.ht
  m
• http//www.merck.com/pubs/mmanual/sections.htm
• Immunology, sixth edition, Roitt.,Brostoff,Male
  2001
• Manual of immunology, Rose and Friedman, 1976

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make your children SMART

• Juan Enriquez