3D reconstruction from confocal microscopy data

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3D reconstruction from confocalmicroscopy data
Alex Sossick Wellcome/CRC Institute University
of Cambridge
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A brief history

• Interest in magnification has been around for a
  long time,
• 2nd Century BC Claudius Ptolemy
• Described a stick appearing to bend in a pool of
  water, and accurately recorded the angles to
  within 1/2 degree for this relationship. He then
  very accurately calculated the refraction
  constant of water.
• 1st Century AD Seneca
• Described actual magnification by a globe of
  water. He wrote the following "Letters, however
  small and indistinct, are seen enlarged and more
  clearly through a globe of glass filled with
  water."
• Specticals were reinvented circa 1280-1285,
• (the Chinese already cosmetically had invented
  specticals by along time before this)

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A brief history

• Credit for the first microscope is usually given
  to Zacharias Jansen, in Middleburg, Holland,
  around the year 1595.
• By the 1880s the theoretical resolving power was
  achieved, 2 points only 0.2 microns apart.
• First production confocal late 1980s

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What is Confocal Microscopy?

• It is similar to conventional epi-illumination
  light microscopy.
• The confocal pinhole removes out of focus light,
  therefore optical sectioning is possible.
• The main advantage is the ability to optical
  section through a sample.
• It is non-invasive

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A Confocal Microscopy
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Nipkow Confocal
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Nipkow Confocal
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Why 3D reconstruction?

• Alot more structural information can be gained
  from looking at a sample at different view points.

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AVS/EXPRESS

• AVS/Express gives a flexible approach to 3D image
  processing

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The Video!!!
A Wellcome/CRC production
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The future with AVS/Express

• Better 3D Imaging
• Segmentation
• 4D Image processing
• Projections through time
• Slices through time

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A function of Z through time
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The people who did the work!
Andrea Brand Jim Hasloff Emma Dormand Isabel
Palacios