Juvenile sturgeon exhibit reduced physiological responses to exercise

1
Juvenile sturgeon exhibit reduced physiological
responses to exercise

• Presented by Misho Borissov
• Kieffer, J., A. M. Wakefield, and M. K. Litvak.
  2001. Juvenile sturgeon exhibit reduced
  physiological responses to exercise. Journal of
  Experimental Biology 204 4281-4289.

2
Foreword
Acipenser brevirostrum
Acipenser oxyrhynchus

• Physiological responses to exercise in juvenile
  sturgeons
• Atlantic Sturgeons
• Short-nose Sturgeons
• Measurement of oxygen consumption, ammonia
  excretion, lactacte, glycogen, pyruvate,
  phosphocreatine, and glucose

3
Oxygen consumption measurements

• Water samples taken from respirometers
• Before exhaustion
• After exhaustion

4
Pictorial representation of oxygen consumption
levels
5
Discussion of the graph aforementioned

• Support and comparison to other fish
• Salmon
• Rainbow Trout
• Siberian Sturgeon

6
Ammonia excretion rate measurements

• Water samples measured
• Pre-exercise ( 5 min before exercise)
• Post-exercise (different time levels after
  exercise)

7
Pictorial representation of the ammonia excretion
rates
Rate of ammonia Excretion (micromole kg X h

8
Discussion of the graph aforementioned

• Comparison to other fish
• Rainbow Trout

9
Metabolite levels and muscle fuel in Acipenser
brevirostrum

• Juvenile sturgeons (18-20 grams in this case)
  used (only the Short-nose Sturgeon used in this
  experiment)
• Muscle and blood removal
• Anesthesia applied so there wont be a great
  acid-base change.

10
Pictorial representation of the muscle fuel and
metabolite levels in the Short nose sturgeon
Lactate
PCr
Pyruvate
Glycogen
Glucose
11
Discussions for the graphs aforementioned

• Explanation for the rapid recovery of sturgeons
• Little metabolic disturbance
• The explanation above can be said to explain the
  rapid recovery of the metabolites.
• Oxygen consumption chart supports this

12
Plasma metabolites in the Short-nose Sturgeon

• Gathering of plasma
• Anesthesia again applied
• Measurement of osmolality
• - number of particles per kilogram of water

13
Pictorial representation of the plasma metabolite
levels in Short nose Sturgeons
14
Discussion of the graph aforementioned

• Comparison with other fish
• Teleost comparison
• Plasma post-exercise lactate levels quickly
  returned to normal.
• In terms of osmolality, no ionic disturbance,
  which means osmoregulatory function is not
  modified with exercise

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Conclusion

• Results Discussed
• Sturgeons do not show a great stress response
• Comparison to other fish suggests that
• Different exercise protocols needed to establish
  new results to support data
• Different water quality needed to establish enw
  results to support data