Coupling of ox

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Coupling of ox phos observed isolated
mitochondria
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DNP uncouples ox phos
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Haloarchea
halo.umbi.umd.edu/haloed/Physiology.htm
bacteriorhodopsin
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bacteriorhodopsin
Haloarchea
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Racker Stoeckenius, 1974 Minimal
reconstitution experiment
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(No Transcript)
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ATP synthase FoF1 ATPase
DG for ADP Pi ? ATP 0 at active site of
synthase
(one conformation of active site)
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ATP synthase structure
F1
F1
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Side views ?
Top bottom views ?
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ATP synthesis animation
http//multimedia.mcb.harvard.edu/
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Conformational coupling
Rotational catalysis
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an experiment to see the motor in action
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3
per NADH 10 H out per ATP 4 H in ? 2.5
ATP/NADH
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Using cytosolic NADH electrons
overall NADHout ? FADH2
overall NADHout ? NADHin
(1.5 ATP)
(2.5 ATP)
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UCPs All mammals except pigs UCP-1 heat
production in brown fat UCP-2 -3 ???????
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(No Transcript)
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Mitochondrion apoptosis Stress ? leaky mito
memb ? cyt C in cytosol
cell death
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Minimizing oxidative stress