Emission Spectroscopy Biochemical Fluorescence Theory, Applications

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Emission Spectroscopy -Biochemical
FluorescenceTheory, Applications Special
TopicsPart I - Fluorescence FundamentalsTuesda
y, May 14, 2002 Kevin T. Schlueter, Ph. D.
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Emission Event Lifetimes
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Morse Curves
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Energy Level Diagram
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Jablonski Diagram
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Fluorometer Layout Diagram
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Ideal Properties of a Spectrofluorometer
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High-Pressure Xenon Arc Lamp
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Source Spectral Outputs
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Typical Source Outputs
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Source Output Correction
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PMTs and PDAs
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Photosensors - Photocathode Materials
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Photosensors - Photomultipliers
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Sampling - Optical Configurations
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Autoabsorption Interferenceat either/or both
Excitation and Emission WavelengthsThe Inner
Filter Effect
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The Inner Filter Effect
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Standards - Emission Intensity Quantum Yield
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Fluorophore Photobleaching
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Fluorophore EnvironmentRearrangement of
Molecular Orbital and Solvent Dipoles
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Reciprocal Transitions and the Mirror Image Rule
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Effect of Solvent Dipolar Relaxation of Excited
State
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Fluorescence Scanning
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Protein Emission Scan - Typical
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3D Emission Scanning
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Intrinsic Fluorophores
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Green Fluorescent Protein(and Orange, and Red,
And Yellow)
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End - Part I