Title: Cypriniformes Tree of Life Initiative
1Cypriniformes Tree of Life Initiative
Richard L. Mayden Integrated Genomics,
Biodiversity and Conservation
Department of Biology Saint Louis
University St. Louis, Missouri 63103 USA
maydenrl_at_slu.edu
www.cypriniformes.org
2Maydens research and laboratory
- Integrated Genomics, Biodiversity, and
Conservation - Species discovery, inventory, descriptions
- Data integration, archiving, concatenation
- Phylogeny reconstruction
- Species concepts and ID methods
- DNA and vouchered specimens for nearly all North
American freshwater species ( 1100 sp.) - Frozen and EtOH
- Photovouchers
- multiple populations and individuals
3- Order Cypriniformes is a major group of
Actiniopterygian fishes - Largest clade of freshwater fishes in the world
expected 5000 species - Cypriniformes are of great importance in
comparative biological studies, aquaculture, and
the pet trade. - Studies on the zebra fish (Danio rerio) are key
to understanding the molecular interactions of
development and developmental evolution - Large carp have been maintained in complex
polyculture systems for thousands of years - Important model organisms in evolution, molecular
evolution, biogeography speciation, comparative
ecology, etc.
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5Issues
- Despite their tremendous importance in human
culture, their diversity, model organisms, and
importance in ecosystem and evolutionary studies
- - basic phylogenetic relationships and diversity
of major clades poorly known, - classifications are problematic,
- many undescribed and cryptic species,
- several areas of planet are poorly inventoried.
6CToL Project Success
- Tree of Life Projects are Unique
- Globally distributed clades
- Participants must think differently and work
together as as community - like superorganism - CToL will be a model project for new way
ofdoing large-scale systematics around World - Participants developed charter for
collaborative work and sharing specimens,
data, authorship, and ideas to achieve our
overall goals of project
7Phylogenetic Objectives
Estimated 3,500 described species
8Levels of Collaboration
9Core Participants Global Geographic Coverage
Great Availability of Taxa
- USA Etheopia South Africa Turkmenistan
- Richard Mayden Abebe Getahun Paul Skelton
Vlaidmir Salnikov - Robert Wood
- Nevin Aspinwall Japan
- Phillip Harris Misaki Miya
- Paula Mabee Kenji Saitoh
- Miles Coburn Mutsumi Nishida
- Andrew Simons
- Hank Bart Mexico
- Nelson Rios Hector Espinosa
- Gloria Arratia Paco Garcia de Leon
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- China Poland
- Huanzhang Liu Jolanta Szlachciak
- Meemann Chang
- Shunping He Russia
- Jun-Xing Yang Nina Bogutskaya
- Alexander Golubtsov
- Alexander Naseka
Associate and Adjunct Participants not listed
10Shared Collaboration -- In concert with
Individual Laboratories Working Independently!
All work together for accomplishing the
1,000 species PHYLOGENY and species
diversity by collaboratories!
AND EACH laboratory can work
separate OR together on othertargeted groups
or taxonomic issues
USA
China
Europe
Russia
Africa
11DNA Identification - A Process
12DNA Barcoding
NuclearGenes
Cyt-B12S16S ND4-5 ND2 CR
MitochondrialGenes
13Web Portal
- Provides an interface for users to query and add
data to the back-end databases - Provides information on resources (tissues,
specimens etc.) available to researchers - Promotes community interaction through shared
workspaces - File repository of various information
- Email/discussion and archives
- Selected content made available to
education/outreach activities
14Online Collaborative Environment for Taxonomic
Research
CTOL Members
Database SQL 2000
Web Portal IIS, Win 2003
Outreach Service
General Public
15Data Objects
- Sequences (ATGCCCTACAGGATC)
- Images (Specimens/Anatomical Parts)
- Trees
- Nexus Files
16Interactive File Management
17Data Entry
18Data Query
19Query Results
20Query Results Detail
21Query Results Detail
22Study Dependent on SharingData, Results, Ideas,
Materials
23Study Dependent on SharingData, Results, Ideas,
Materials
24Welcome to CToL
- The CToL welcomes collaborators to the project
- Collaborators can contribute in many ways
- Data generation - Taxon
Sampling - Morphological characters - Molecular
characters - Web resources - Species
sampling - Collaborators are authors or credited for their
contributions - We have limited money to distribute but seek
proposals - Annual meetings at ASIH
- Finally, hopefully we can help you obtain support
for this international effort from different
science agencies of countries, other agencies,
foundations, etc.! - Congratulations to Japanese collaborators for
funded Cypriniformes research following NSF Tree
of Life