MCB 407

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MCB 407 Thursday Aug 23 2007 Cell Biology of
Neurons Cell Polarity Organelles Molecular
Dogma Protein Translation Cytoskeleton Axonal
Transport Membranes
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Section 1A David Andrew Thursday 5-550 Room
240 LSS Al-awadhi, Saud Buzzetti, Robert Cox,
Laurel Dahlstrom, Marcus Delgado, Ryan Dutson,
Karl Eleid, Joseph Frisby,Jovanca Itsuno,Jaunette
Lopez,Katherine Mosher, Clayton Orologio,
Emily Panagos, Melissa Post, Tracy Reeves,
Christine Shah, Amelia Smillie, Chris Underwood,
Alan Workinger, Sarah
Section 1B Nadia Corel-Frias Thursday 5-550
Room 340 LSS Cardwell, Brian Cushing,
Melanie Davies, Kelli Diaz, Eduardo Edwards,
Drew Forbes, Kathryn Hollinger, Patrick Kimbro,
Joseph O'Brien, Jesse Ortega, Michael Pike,
Sarah-ann Raybuck, Brendan Russo, Gary Shir,
Ahmad Torres, Norma Wilford, Erwin Zaninovich,
Orel Datta, Neha
Section 2A Thabelo Khoboko Friday 3-350 Room
210 Bio West Brown, Jonathan Dave, Ami Feltz,
Jillian Heintz, Achin Kim, Jennifer Kramer,
Joshua Luginbill, Amy Martinez,
Lindsey Mclaughlin, Michael Nabiyouni,
Maryam Okun, Alec Reavis, Daniel Soap,
Julia Strohen, Monica Thom, Nicole Vermeer,
Adrian Washington, Theron Wiede, Ashley
Section 2B Melinda Mittelman Friday 3-350 Room
240 LSS Clements, Elizabeth Duart,
Jessica Gibboni, Robert Kersten, Jillian Kim,
Jonathan Lacy, Marian Malhotra, Kyle Mitchell,
Molly Nagatomo, Kei Pal, Prasun Shuey,
Megan Stombaugh, David Sylvester,
Deanna Tonsfeldt, Karen Vomocil, Elisa Wick,
Shanti Strobel, Stephanie
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FIG 4.1
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FIG 4.1
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MCB 4.1
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MCB 16-1
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FIG 4.5
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MBOC19.6
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MBOC19.6
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MBOC 20.7
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Growth Cone Movie MICROTUBULE MOVIE
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MBOC 20.2
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MCB 20.21
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MCB20.24
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ER TO GOLGI
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MBOC19.33
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MCB19.35
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Kandel 4-6
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Fig 5-5
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Fig 5-7
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MBOC 17-1
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Fig 5-11
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MBOC20.17
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MBOC20.17
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AXONAL TRANSPORT MOVIE
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In class Exercise

• Why is axonal transport more efficient in axons
  than dendrites?
• 2. What motor protein(s) are responsible for fast
  and/or
• slow axonal transport?
• 3. Design an experiment to test your idea.

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Cell plasma membranein the transmission
electron microscope (TEM)? 60-80 Å (6-8 nm)
thick? 2 25-Å electron-dense layers?
1 30-Å electron-lucent layer
chemical composition ? 50 lipid ? the rest
protein and a small amount of carbohydrate
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Smith fig 6.1
Principal membrane lipids glycerophospholipids
A schematic B phosphatidyl choline C
phosphatidyl ethanolamine D phosphatidyl
serine E phosphatidyl inositol
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Membrane lipids
hydrophilic
hydrophobic
hydrophilic
Phospholipid bilayer
mainly ? glycerophospholipids
(phospholipids) ? cholesterol
aqueous environment
Phospholipid micelle
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Artificial lipid bilayer membrane model of Müller
Rudin
phospholipids in solvent
phospholipid bilayer formed at pin-hole in a
partition separating 2 aqueous solutions
black lipid bilayer named for its black
interference color
solvent diffusion
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Physical properties of membranes (based on
Müller Rudin black lipid bilayer model
membrane) cell membrane lipid
bilayer capacitance 1 µF/cm2 1
µF/cm2 resistance 103 O cm2 106109 O
cm2 H2O permeability 90400 µm/sec 17104
µm/sec surface tension 0.31 dyne/cm 0.52
dyne/cm
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Cooper Fig 2.49
Permeability of phospholipid bilayers
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Cooper fig 2.48
Fluid mosaic model of membrane structure
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Nicholls Fig. 2.1 (A)
Ion channels in the sea of the membrane
phospholipid bilayer