A1262529188HqyZw

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• Protocol for ELISA Sample Submission-96 well
  General Procedures
• Primary assays x-40 x-42. (1-40, 1-42,
  1-total on special request).
• Linear range 1pM (4pg/ml) - 110pM (500pg/ml).
• 310ul/well is needed for each assay. If both
  x-40 and x-42, then submit two plates, one for
  each assay.
• 2G3 and 21F12 capture antibodies used for x-40
  and x-42 assays, respectively 266B antibody used
  for detection.
• Submit samples on 96 well plate!! Use Storage 96
  well plates that hold 500 ul/well.
• Please DO NOT use tissue culture plates or plates
  that do not hold more than 500ul/well.
• Please leave 2 rows empty for peptide standard.
• Always submit a submission sheet ahead of time.
  Even if you talk to Jackie about preparing a
  submission, you should submit a sheet at that
  time. This is the ONLY way that Jackie can plan
  ahead and prepare for assays. Submission sheets
  can be emailed to Jackie or dropped off at
  Jackies desk.

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Protocol for ELISA Sample Submission-triplicate
sample layout

• --For 1 assay submit 310ul/sample for 2
  assays(x-40 x-42) submit two plates, each with
  310ul/sample
• --Add samples to a 96 well plate, starting at C1
  and moving across, as shown on left below.
• --Fill 4 wells in each row before starting
  another row.
• --Fill up to 6 rows.

Your sample layout (on 96 well plate)
1 2 3 4 5 6 7
8 9 10 11 12
A B C D E F G H
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Example of sample submission sheet 96 well
triplicate
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Results will be sent to you via e-mail in the
following format
Triplicate
Standards-top two rows in yellow Blank
controls-highlighted in gray Conditioned media
controls and plasma controls-between blank
controls Your samples-will all be highlighted in
one color with your name next to the samples