Primal

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Primal
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H A P
HAP

• HAP hypothalamus, amygdala, pituitary
• 2 of brain mass
• Instinctive and emotional behaviors

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GPCRs

• Receptors on surface of cells
• Control functions in brain, heart, lung
• Majority of GPCRs in brain
• HAP has highest number
• Associated with behaviors
• Excellent drug targets
• 45 of drugs react with 10 of GPCRs

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Drugs Marketed Worldwide
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Rapid Gene KO

• KO GPCRs in embryo (ES) cell

• Create libraries of ES cells
• 10 million cells

• Identify specific GPCR Kos
• 211 KO GPCRs detected

• Generate mice from mutant ES cells
• 81 different mutant mice produced (40)

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Primals Strategy
GPRC Isolation
Rapid KOs
Behavioral Tests
Animal Screen
Drug Screen
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Primals Pipeline
2004
2003
2001
2002
Q4-02 Q4-03 Q4-04 Cloned Genes 40 100 100 H
ybrid Animals 40 100 100 Mutant
Animals 20 80 100 KO Mice 7 62 100 Drug
Targets None 25 65 Ligands/Drugs None 2 20
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Intellectual Property
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GPCR Patents