PV92 Insert Lab

1
PV92 Insert Lab

• Make sure the gels are in lab note books! I sent
  them to pingry email on Friday. Print out now!
• After presentation mark genotype of each person
  that has a lane.

2
All Alus are approximately 300 bp in length and
derive their name from a single recognition site
for the restriction enzyme AluI located near the
middle of the Alu element. Human chromosomes
contain about 1,000,000 Alu copies, which equal
10 of the total genome. Alu elements probably
arose from a gene that codes for RNA signal
peptide for export
3
Alu Insert

• Alu elements are found only in primates the
  "monkey" branch of the evolutionary tree, which
  includes humans. So, all of the hundreds of
  thousands of Alu copies have accumulated in
  primates since their separation from other
  vertebrate groups about 65 million years ago.
• Once an Alu inserts at a chromosome locus, it can
  copy itself for transposition, but there is no
  evidence that it is ever excised or lost from a
  chromosome locus. So, each Alu insertion is
  stable through evolutionary time. Each is the
  "fossil" of a unique transposition event that
  occurred only once in primate evolution. Like
  genes, Alu insertions are inherited in a
  Mendelian.
• An estimated 500-2,000 Alu elements are
  restricted to the human genome. The vast majority
  of Alu insertions occur in non-coding region.
  However, an Alu insertion in the NF-1 gene is
  responsible for neurofibromatosis I.

4
Determine the Genotype of Each Student . -,
--
5
Genotypes of Students