Phage Deposition/TEM

1
Phage Deposition/TEM

• Mod3 Day5
• Thursday Nov 20th

2
Electrodeposition
3
Part 1

• Clean your patterned slide as on Tues
• Mix 50 ml IrOx with 1 ml phage
• Electrodeposit 10 minutes 7.5V
• Mix sample
• Electrodeposit 10-20 minutes more
• Should see purple pattern
• Store in 50 ml conical in your drawer

4
TEM
5
Part 2

• Mix equal volumes of your phage IrOx
• Holding grid by edge with tweezers, add 15 ul of
  IrOx phage
• Rest 30 undisturbed
• Pipet to remove volume
• Wash with 15 ul H2O (pipet on/off)
• Walk to 13-1012 to meet Mark Allen

6
FNT

• FNT Dec 2nd!
• One question about ECD
• Time to work on your proposals
• Time to work on your re-write Mod2
• Almost done!!