Normal Cartilage

1
Normal Cartilage
-Light micrograph of human articular cartilage
from the medial tibial plateau of a 45-year old
man. Weigerts acid iron chloride hematoxylin,
safranin-O and fast green stain (proteoglycan red
and collagen green)
Provided by Y Henrotin
2
Normal cartilage
B
A
C

• Normal cartilage with chondrocytes isolated in an
  extended
• extracellular matrix (A). The calcified cartilage
  layer overlying subchondral bone
• (B). Normal subchondral bone (C).

Provided by Y Henrotin
3
Chondrocyte clusters in OA cartilage

• Chondrocyte clusters (arrows) are visible in the
  fibrillated
• superficial layer of OA cartilage

Provided by Y Henrotin
4
Subchondral bone sclerosis

• Subchondral bone sclerosis (arrow) is
  characterized by trabecular
• thickening, low mineralized osteoid substance
  accumulation and
• osteoblasts phenotype alteration

Provided by Y Henrotin
5
Multiple Tidemark
- Multiple tidemark are visible in OA calcified
cartilage (multiple arrows)
Provided by Y Henrotin
6
Osteochondral plate microcracks
A

• Subchondral bone sclerosis (A). Multiple
  microcracks
• are visible at the bone/cartilage junctions
  (arrows)

Provided by Y Henrotin