By Angela Horst

1
Antimicrobial effect of Metal Oxide Nanoparticles

• By Angela Horst
• PI Dr. Yinjie Tang
• Mentor Dr. Bing Wu

2
What

• 6 Metal Oxides vs. Escherichia coli
• ZnO
• Co3O4
• CuO
• NiO
• Fe2O3
• Fe3O4
• 2 Exposure Methods
• Aqueous
• Electrospray

3
Why Metal Oxides

• Uses in industry
• Semiconductors
• Pigment and Sunscreen
• Solar Panels
• Drug Delivery
• More
• Negative effects on environment
• Harmful to humans
• Harmful to ecosystems

4
Why Two Methods

• Aqueous
• Mimics ecosystem
• Limits exposure
• Electrospray
• Provides easier control
• Allows direct contact

5
Methods Explained

• Aqueous

• 2, 20, 100 mg/L
• M9 Minimal Media
• 3, 6, 9, 21, 24 hour

• Electrospray

• Inoculate Membrane
• Electrospray Biofilm
• Recover as Aqueous

6
Results
Ionic Copper

• Copper (II) Oxide

Electrospray
7
Conclusion

• Aqueous toxicity attributed to ions
• Aggregation prevents aqueous toxicity
• Nanoparticle effect seen
• CuO
• Co3O4
• ZnO

8
References