DNA

1
Central Dogma of Biology
DNA
RNA
Protein
2
Coupling between different steps and dynamic mRNP
Transcription
3
Post-transcriptional Steps of Gene Regulation
- Capping
- Splicing cis, trans, alternative
- Polyadenylation
- Modifications - base and sugar
- Editing
- Export/Import
- Stability
- Localization
- Translation
4
R-Loop Experiment Reveals Introns
5
Splicing A Process to Remove Introns
6
Splicing Signals
Major introns
Y80NY80Y87R75A100Y95
Branch site
7
Simplified Mechanism of Nuclear mRNA Splicing
8
Splicing intermediates
9
The Spliceosome Cycle
Human 60S Yeast 40S
10
U1 snRNA Base-pairs with 5 Splice Site
11
U2 snRNA Base-pairs with Branch Site
12
A Stable RNA-RNA Base-pairing Interaction
13
ATP and DEAD-box Proteins Participate in RNA-RNA
rearrangements
14
RS Domain Promotes RNA-RNA Annealing
15
Discovery of Ribozyme
Tetrahymena Group I intron
16
Mechanism of Group I Splicing
17
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