Restriction Maps DNA '

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Lecture 13

• ???? ?? ????? ??? Restriction Maps (???? ??
  ????? ?-DNA ??????? ??
  ?????
  ?????). 

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• ?????? ??????
• 1. RESTRICTION MAPS
• 1.1 Restriction enzymes.
• 1.2 Palindromes in biology.
• 1.3 Restriction maps show the location or
  location
• of restriction sites along linear or circular
  DNA.
• 1.4 Interval graphs.
• 1.4.1 Example.

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H ?????? ????????? - ????? ?? ????????
????????? ??? ??? ????? ?? ???? ??????? ??????
????? ????? ??? ???? ????? ??? ?????
?????. ????? - ??? ????? ????? ?? ?????
??????? (???????????) ?? DNA ????? ???????.
?????? , GAATTC CTTAAG
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???? ?????? ???? ?300- ????? ?? Restriction
Enzymes ??????? ?????? ?''? ?? ??????? ?? ??
????? ????? ?????? ????? , ???? , ,
HaeIII ???? ???? ?? ??????? ???????? ?? ???
Haemophilus aegyptius ?- III- ??? ?????? ??
?????? ????? ????????.
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H ????? ?????? ?? DNA ?''? ?????

• ???? ??? ???? ?????? ?? ????? ?? DNA ?''? ?
  ???????.
• ?. ???? ?????? ??? ???? Blunt Ends , ?????? ,

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????? ???? ???? ?? ???? ???????? ?????? ??????
??? ?? ??????.
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• ?????

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???? ????? ?????? ????? ?? ?????? ???????? A ?-B
?? ??? ?? .DNA
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H ???????? ?????? Restriction Map
Set L0 L. 1. Find an end. Find L0
? v such that only one adjacent L0 ? u. If no
such u exists, go to step 3. 2. Find connected
component. Set v1 v and v2 u . Extend the
sequence of edges ? v1,v2 ?,? v2,v3 ?, ,?
vr-1,vr ? to maximum r , where all L0 ?
vi. Remove ?v1, , vr ? from L0. Go to step
1. 3. Fill in the component. For L? L0 ? v
and L ? u with E ?? u,v? put ?v,u? between
?vi,v? and in the component edge list.
4. Single intervals. For L0 ? v for all L
? ui with E ?? ui,v? add ?u1,v??u2,v ? to
list of components. 5. Isolated pairs. For E
? ?v,u?with L? v,u add the edge ?v,u? to the
list of components. 6. Done.
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