AML1/Runx1 and oncogenic transformation

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AML1/Runx1 and oncogenic transformation
RUNT domain (DNA BINDING)
Chr 21
Chr 8
4 Nervy repressor domains
Translocation (821)
In human myeloid leukemia gt
RUNT domain
4 Nervy repressor domains
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Mcoffee multiple sequence alignment and
phylogenetic tree
RUNX1 alignment
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(Drosophila)
NO alignment between species for the t(821)
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Is there a difference between the proteinic
folding of AML1 and AML1-ETO??
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Predicted structures of AML1-ETO domains
NCBI- BLAST
RUNT
Zf
NRH2 domain
PDB- models database
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AML1-ETO hydrophobic cluster analysis with
drawhca
Runt
TAFH(NHR1)
NHR2
NHR3
zf-NHR4
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Scanning for Phosphorylation-sites of AML1
Scansite
ERK-1
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Conclusions

• Biocomputing study of AML-1 ETO
• Sequences comparison vs AML-1
• 3D prediction
• Hydrophobic domains prediction
• Potential phosphorylation sites of AML1
• Comparison with AML1-ETO

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• .....and now you have to return to the lab and
  make experiments...........