Gel Electrophoresis

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Gel Electrophoresis

• By
• Vijal Shah
• Raziya Charaniya

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What is Gel Electrophoresis?

• A technique used for separation of fragments
  based on size and charge

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How does it work?

• Electric field is applied
• The charged molecules migrate across the gel
• The Gel acts as a sieve
• Separation can be based on size
• Smaller molecules travel farther
• Separation can be based on charge
• Negatively charged molecules travel towards the
  positive terminal

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Steps for Gel Electrophoresis

• Gel Preparation
• Set up the gel apparatus
• Load the sample into the gel
• Hook up the electric current and run the gel
• Stain the gel and analyze the results

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Gel Preparation
www.worldofteaching.com
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Steps for Gel Electrophoresis

• Make the Gel
• Set up the gel apparatus
• Load the sample into the gel
• Hook up the electric current and run the gel
• Stain the gel and analyze the results

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Setting up the apparatus
www.electrophoresis.co.uk
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Steps for Gel Electrophoresis

• Make the Gel
• Set up the gel apparatus
• Load the sample into the gel
• Hook up the electric current and run the gel
• Stain the gel and analyze the results

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Load the sample into the gel
http//www.flutrackers.com/forum/showthread.php?t
11286
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Steps for Gel Electrophoresis

• Make the Gel
• Set up the gel apparatus
• Load the sample into the gel
• Hook up the electric current and run the gel
• Stain the gel and analyze the results

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Hook up electric current and run the gel
http//commons.wikimedia.org/wiki/FileGel_electro
phoresis_apparatus.JPG
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Steps for Gel Electrophoresis

• Make the Gel
• Set up the gel apparatus
• Load the sample into the gel
• Hook up the electric current and run the gel
• Stain the gel and analyze the results

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Stain the gel and analyze results
http//a-s.clayton.edu/fnorflus/BIOL4202L/Spring20
09/couseschedulesp2009.htm
http//commons.wikimedia.org/wiki/FileGel_electro
phoresis_2.jpg
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Lets Visualize!

• Gel Electrophoresis Virtual Lab

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More Applications

• Proteins
• Gel electrophoresis is used in forensics,
  molecular biology, genetics, microbiology and
  biochemistry.

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Thank you for your patience- Vijal ShahRaziya
Charaniya