TRP OPERON

1
TRP OPERON

• THE TRP OPERON CONTAINS 5 STRUCTURAL GENES, TRP
  A,B,C,D,E. THESE GENES ENCODE FOR 3 ENZYMES THAT
  TURN CHORISMIC ACID INTO TRYPTOPHAN.

2
TRP E-Anthranilate synthetase I

• Converts chorismate to anthranilate using
  ammonia.
• Anthranilate is an amino acid that is later
  broken down into another amino acid, Trysoine.

3
TRP D-Anthranilate synthetase II

• A type of Glycosyltransferase that participates
  in the synthesis of Tryptophan
• Produces an amino acid called Anthranilate which
  ,by the help of TRP E, is broken down into
  another amino acid, Trysoine.
• Converts -(5-phospho-D-ribosyl)-anthranilate and
  diphosphate to anthranilate and
  5-phospho-alpha-D-ribose 1-diphosphate.
• It is 1 of the 2 components that together encode
  Anthranilate synthetase

4
TRP C- indole-3-glycerol-phosphate synthase

• Involved in the synthesis of tryptophan and amino
  acids.
• It is a type of lyase that cleaves carbon-carbon
  bond.
• this ability allows it to converts
  1-(2-carboxyphenylamino)-1-deoxy-D-ribulose
  5-phosphate to C(1)-(3-indolyl)-glycerol
  3-phosphate and carbon dioxide and water

5
TRP B tryptophan synthase subunit B

• There are two beta subunits which form a dimer
• Beta dimer catalyzes the formation of
  L-tryptophan from L-serine and 1-(indol-3-yl)glyce
  rol 3-phosphate

6
TRP A tryptophan synthase subunit A

• Tryptophan synthase is an enzyme found in plants
  and bacteria
• There are two separate alpha subunits
• They catalyze the formation of indole and
  glyceraldehyde 3-phosphate from indoleglycerol
  phosphate in tryptophan biosynthesis, which is
  needed by the B subunits.