Gene Expression II: RNA Processing

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Gene Expression II RNA Processing
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Splice Signals
Donor
Acceptor
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Splicing Events
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Three Part Junction
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Trans-Splicing
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Trans-Splicing
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RNA-RNA Interactions
Remember the SnRNPs?
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Spliceosome-Mediated Splicing
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Group I Introns into Ribozymes
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Group I and II Introns
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RNA as an Enzyme
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Thomas R. Cech
Distinguished Professor and Investigator, Howard
Hughes Medical Institute (biochemistry). Ph.D.,
University of California, Berkeley, 1975 USPHS
Research Career Development Award, 1980 Pfizer
Award in Enzyme Chemistry, 1985 Guggenheim
Fellow, 1985 American Cancer Society Lifetime
Research Professor, 1986 U. S. Steel Award in
Molecular Biology, 1987 Member National Academy
of Sciences, 1987 Heineken Prize, 1988 Nobel
Prize in Chemistry, 1989 National Medal of
Science, 1995.
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Alternative Splicing I
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Alternative Splicing II
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Alternative Splicing III
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Splicing Errors
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SR Proteins Recruit Spliceosome Components
SR is serine (S) arginine (R) rich
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Five Alternative Splice Products
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SV40 Large T Antigen
Two forms routinely produced.
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Regulation of Alternative Splicing
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hnRNPi Inhibits Splicing
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Adenovirus 2 Genome
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Locating Genes in Adenovirus
R-loop mapping of late mRNAs
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Spliceosome Catalyzed Splicing
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Conserved Sequences in Different Introns
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Different Exons, Different Domains
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Mix and Match Parts I
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Mix and Match Parts II
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Evolution of a Protein Family
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ABC Gene Family and Human Disease
  Stargardt/FFM, RP, CRD, CD Ivermectin
susceptibility Immune deficiency Immune
deficiency PFIC3 XLSA/A PFIC2 Dubin-Johnson
Syndrome Pseudoxanthoma elasticum Cystic
Fibrosis, CBAVD FPHHI ALD Sitosterolemia Sitostero
lemia
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RNA Editing by Deamination
Cytidine deaminase changes C ? U
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RNA Editing by Deamination
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RNA Editing in Disease
RNA processing and clinical variability in
neurofibromatosis type I (NF1) Gary R. Skuse and
Amedeo J. Cappione
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Guide-Mediated RNA Editing
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mRNA Transport to Cytoplasm