Chapter 9 Regulation of Gene Expression

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Chapter 9 Regulation of Gene Expression
Sensing and Signal Transduction
Regulation of Transcription
Global Regulation
RNA Based Regulation
Regulating Development
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FINAL ROUND
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Topic 1 Regulation of Transcription100 Question

• Question Which of the following is/are true
  about regulatory proteins that interact with DNA?
  
• a. they interact at the major groove
• b. they typically bind at inverted repeats in
  the DNA
• c. they are typically homodimeric
• d. all of the above

ANSWER
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Topic 1 Regulation of Transcription 100 Answer

• Question Which of the following is/are true
  about regulatory proteins that interact with DNA?
  
• a. they interact at the major groove
• b. they typically bind at inverted repeats in
  the DNA
• c. they are typically homodimeric
• d. all of the above

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Topic 1 Regulation of Transcription 200
Question

• Question The transcription of genes for enzymes
  used in anabolic pathways are typically regulated
  via induction.
• a. true
• b. false

ANSWER
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Topic 1 Regulation of Transcription 200 Answer

• Question The transcription of genes for enzymes
  used in anabolic pathways are typically regulated
  via induction.
• a. true
• b. false

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Topic 1 Regulation of Transcription 300
Question

• Question Which is true when arginine is in
  excess?
• a. arginine binds to the operator
• b. arginine binds to the repressor
• c. the repressor detaches from the operator
• d. the genes in the arginine operon are fully
  transcribed

ANSWER
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Topic 1 Regulation of Transcription 300 Answer

• Question Which is true when arginine is in
  excess?
• a. arginine binds to the operator
• b. arginine binds to the repressor
• c. the repressor detaches from the operator
• d. the genes in the arginine operon are fully
  transcribed.

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Topic 1 Regulation of Transcription 400
Question

• Question When an inducer binds to the repressor,
  _______________.
• a. the genes are transcribed
• b. the genes are not transcribed
• c. the repressor binds to the operator
• d. the repressor binds to the promoter

ANSWER
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Topic 1 Regulation of Transcription 400 Answer

• Question When an inducer binds to the repressor,
  _______________.
• a. the genes are transcribed
• b. the genes are not transcribed
• c. the repressor binds to the operator
• d. the repressor binds to the promoter

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Topic 1 Regulation of Transcription 500
Question

• Question Which is an example of a gene that has
  constitutive expression?
• a. beta galactosidase
• b. an arginine biosynthesis enzyme
• c. a maltose catabolic enzyme
• d. none of the above

ANSWER
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Topic 1 Regulation of Transcription 500 Answer

• Question Which is an example of a gene that has
  constitutive expression?
• a. beta galactosidase
• b. an arginine biosynthesis enzyme
• c. a maltose catabolic enzyme
• d. none of the above

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Topic 2 Sensing and Signal Transduction100
Question

• Question Which amino acid residue on the surface
  of the cell is typically phophorylated by the
  sensor kinase protein?
• a. tryptophan
• b. arginine
• c. glycine
• d. histidine

ANSWER
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Topic 2 Sensing and Signal Transduction 100
Answer

• Question Which amino acid residue on the surface
  of the cell is typically phophorylated by the
  sensor kinase protein?
• a. tryptophan
• b. arginine
• c. glycine
• d. histidine

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Topic 2 Sensing and Signal Transduction 200
Question

• Question For E. coli, when osmotic pressure is
  high, OmpR (response regulator) is
  phosphorylated.
• a. true
• b. false

ANSWER
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Topic 2 Sensing and Signal Transduction 200
Answer

• Question For E. coli, when osmotic pressure is
  high, OmpR (response regulator) is
  phosphorylated.
• a. true
• b. false

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Topic 2 Sensing and Signal Transduction 300
Question

• Question An example of an autoinducer is
  _______________.
• a. lactose
• b. acyl homoserine lactone
• c. glucose
• d. tryptophan

ANSWER
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Topic 2 Sensing and Signal Transduction 300
Answer

• Question An example of an autoinducer is
  _______________.
• a. lactose
• b. acyl homoserine lactone
• c. glucose
• d. tryptophan

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Topic 2 Sensing and Signal Transduction 400
Question

• Question P. aeruginosa uses quorum sensing to
  regulate genes specific for _______________.
• a. bioluminescence
• b. reproduction
• c. biofilm formation
• d. osmotic tolerance

ANSWER
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Topic 2 Sensing and Signal Transduction 400
Answer

• Question P. aeruginosa uses quorem sensing to
  regulate genes specific for _______________.
• a. bioluminescence
• b. reproduction
• c. biofilm formation
• d. osmotic tolerance

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Topic 2 Sensing and Signal Transduction 500
Question

• Question Chemotactic attractants lead to a
  _______________ level of CheY-P, which leads to
  smooth swimming.
• a. lower
• b. higher

ANSWER
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Topic 2 Sensing and Signal Transduction 500
Answer

• Question Chemotactic attractants lead to a
  _______________ level of CheY-P, which leads to
  smooth swimming.
• a. lower
• b. higher

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Topic 3 Global Regulation100 Question

• Question What is true when E. coli is grown in
  media containing glucose?
• a. CRP is bound at the CAP binding site
• b. there is plenty of cyclic AMP available
• c. the lactose operon is fully transcribed
• d. cyclic AMP is not synthesized and is
  transported out of the cell

ANSWER
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Topic 3 Global Regulation100 Answer

• Question What is true when E. coli is grown in
  media containing glucose?
• a. CRP is bound at the CAP binding site
• b. there is plenty of cyclic AMP available
• c. the lactose operon is fully transcribed
• d. cyclic AMP is not synthesized and is
  transported out of the cell

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Topic 3 Global Regulation200 Question

• Question Which of the following is true when E.
  coli is grown in media containing both glucose
  and lactose?
• a. CRP is bound to the CAP binding site.
• b. the lactose repressor is not bound to the
  operator
• c. there is plenty of cyclic AMP to bind to
  CRP
• d. the lactose metabolism genes are fully
  expressed

ANSWER
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Topic 3 Global Regulation200 Answer

• Question Which of the following is true when E.
  coli is grown in media containing both glucose
  and lactose?
• a. CRP is bound to the CAP binding site.
• b. the lactose repressor is not bound to the
  operator
• c. there is plenty of cyclic AMP to bind to
  CRP
• d. the lactose metabolism genes are fully
  expressed

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Topic 3 Global Regulation300 Question

• Question According to the stringent response,
  when a cell is switched from a nutrient-rich
  medium to a nutrient-poor medium, which of the
  following is still synthesized immediately after
  the transfer?
• a. rRNA
• b. tRNA
• c. amino acids
• d. DNA

ANSWER
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Topic 3 Global Regulation300 Answer

• Question According to the stringent response,
  when a cell is switched from a nutrient-rich
  medium to a nutrient-poor medium, which of the
  following is still synthesized immediately after
  the transfer?
• a. rRNA
• b. tRNA
• c. amino acids
• d. DNA

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Topic 3 Global Regulation400 Question

• Question pppGpp is usually synthesized by
  _______________.
• a. RelA
• b. DksA
• c. SpoT
• d. rRNA

ANSWER
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Topic 3 Global Regulation400 Answer

• Question pppGpp is usually synthesized by
  _______________.
• a. RelA
• b. DksA
• c. SpoT
• d. rRNA

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Topic 3 Global Regulation500 Question

• Question In unstressed cells the relative level
  of DnaK is _______________ and the level of
  intact RpoH _______________.
• a. low high
• b. high low
• c. low low
• d. high high

ANSWER
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Topic 3 Global Regulation500 Answer

• Question In unstressed cells the relative level
  of DnaK is _______________ and the level of
  intact RpoH _______________.
• a. low high
• b. high low
• c. low low
• d. high high

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Topic 4 Regulating Development100 Question

• Question Which process stimulates sporulation?
• a. dephosphorylation of CheY
• b. phosphorylation of GroEL
• c. dephosphorylation of the anti-sigma factor
  F
• d. phosphorylation of Spo0A

ANSWER
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Topic 4 Regulating Development100 Answer

• Question Which process stimulates sporulation?
• a. dephosphorylation of CheY
• b. phosphorylation of GroEL
• c. dephosphorylation of the anti-sigma factor
  F
• d. phosphorylation of Spo0A

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Topic 4 Regulating Development200 Question

• Question How many sigma factors are involved in
  sporulation?
• a. 1
• b. 2
• c. 3
• d. more than 3

ANSWER
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Topic 4 Regulating Development200 Answer

• Question How many sigma factors are involved in
  sporulation?
• a. 1
• b. 2
• c. 3
• d. more than 3

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Topic 4 Regulating Development300 Question

• Question How does anti-sigma factor play a role
  in spore-forming cells?
• a. it binds to sF and inactivates it
• b. it binds to Spo0A and inactivates it
• c. it is transcribed into sF
• d. it acts as a toxin, thus killing
  neighboring cells

ANSWER
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Topic 4 Regulating Development300 Answer

• Question How does anti-sigma factor play a role
  in spore-forming cells?
• a. it binds to sF and inactivates it
• b. it binds to Spo0A and inactivates it
• c. it is transcribed into sF
• d. it acts as a toxin, thus killing
  neighboring cells

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Topic 4 Regulating Development400 Question

• Question When CtrA is phosphorylated,
  _______________.
• a. GcrA synthesis is activated
• b. free-swimming swarmer Caulobacter cells
  become stalked cells
• c. Caulobacter cells start to replicate their
  DNA
• d. stalked Caulobacter cells become free-
  swimming swarmer cells

ANSWER
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Topic 4 Regulating Development400 Answer

• Question When CtrA is phosphorylated,
  _______________.
• a. GcrA synthesis is activated
• b. free-swimming swarmer Caulobacter cells
  become stalked cells
• c. Caulobacter cells start to replicate their
  DNA
• d. stalked Caulobacter cells become free-
  swimming swarmer cells

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Topic 4 Regulating Development500 Question

• Question When levels of DnaA rise in
  Caulobacter the cell is switching to its swimming
  form.
• a. true
• b. false

ANSWER
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Topic 4 Regulating Development500 Answer

• Question When levels of DnaA rise in
  Caulobacter the cell is switching to its swimming
  form.
• a. true
• b. false

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Topic 5 RNA Based Regulation 100 Question

• Question What happens to mRNA molecules
  following binding of their antisense RNAs?
• a. the double-stranded RNA is translated much
  better than the single stranded mRNA
• b. ribonucleases degrade the double- stranded
  RNA
• c. the double-stranded RNA forms a secondary
  structural element that is recognized by
  ribosomes
• d. they are reverse transcribed to DNA

ANSWER
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Topic 5 RNA Based Regulation 100 Answer

• Question What happens to mRNA molecules
  following binding of their antisense RNAs?
• a. the double-stranded RNA is translated much
  better than the single stranded mRNA
• b. ribonucleases degrade the double- stranded
  RNA
• c. the double-stranded RNA forms a secondary
  structural element that is recognized by
  ribosomes
• d. they are reverse transcribed to DNA

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Topic 5 RNA Based Regulation 200 Question

• Question Which of the following is true about
  antisense RNA molecules?
• a. they are the same length as their target
  mRNA
• b. they are transcribed from the non-template
  strand of the DNA in the same gene as their
  target mRNA
• c. they are encoded in an anti-gene that is
  found separate from their target mRNA gene
• d. there is no evidence for antisense RNA
  actually enhancing the translation of its
  target mRNA.

ANSWER
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Topic 5 RNA Based Regulation 200 Answer

• Question Which of the following is true about
  antisense RNA molecules?
• a. they are the same length as their target
  mRNA
• b. they are transcribed from the non-template
  strand of the DNA in the same gene as their
  target mRNA
• c. they are encoded in an anti-gene that is
  found separate from their target mRNA gene
• d. there is no evidence for antisense RNA
  actually enhancing the translation of its
  target mRNA.

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Topic 5 RNA Based Regulation 300 Question

• True or False. Some RNA has catalytic activity.
  
• a. true
• b. false

ANSWER
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Topic 5 RNA Based Regulation 300 Answer

• True or False. Some RNA has catalytic activity.
  
• a. true
• b. false

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Topic 5 RNA Based Regulation 400 Question

• Question Riboswitches usually control gene
  expression _______________.
• a. after transcription and translation of the
  gene
• b. after transcription and before translation
  of the gene
• c. before transcription and translation of the
  gene
• d. before transcription and after translation
  of the gene

ANSWER
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Topic 5 RNA Based Regulation 400 Answer

• Question Riboswitches usually control gene
  expression _______________.
• a. after transcription and translation of the
  gene
• b. after transcription and before translation
  of the gene
• c. before transcription and translation of the
  gene
• d. before transcription and after translation
  of the gene

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Topic 5 RNA Based Regulation 500 Question

• Question In E. coli, when tryptophan is in
  excess _______________.
• a. the leader sequence is fully translated
• b. a 3,4 stem loop structure forms in the mRNA
• c. transcription of the tryptophan operon
  terminates early
• d. all of the above

ANSWER
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Topic 5 RNA Based Regulation 500 Answer

• Question In E. coli, when tryptophan is in
  excess _______________.
• a. the leader sequence is fully translated
• b. a 3,4 stem loop structure forms in the mRNA
• c. transcription of the tryptophan operon
  terminates early
• d. all of the above

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FINAL ROUND Question

• Question An example of a positive regulatory
  control is _______________.
• a. the repression of the arginine regulon
• b. the induction of the lactose operon
• c. the activation of the maltose operon
• d. attenuation of the Trp operon

ANSWER
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FINAL ROUND Answer

• Question An example of a positive regulatory
  control is _______________.
• a. the repression of the arginine regulon
• b. the induction of the lactose operon
• c. the activation of the maltose operon
• d. attenuation of the Trp operon

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