Bacterial Transformation

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Bacterial Transformation

• E. Coli
• plasmids
• Restriction Enzymes
• Sticky Ends
• DNA ligase
• Heat Shock
• Transgenic Bacteria
• Recombinant DNA

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Bacterial Transformation
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Bacterial Transformation
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Procedure for Transformation
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DNA Fingerprinting
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DNA Fingerprinting

• Collect Sample
• Isolate DNA
• Digest with Restriction Enzymes
• Get RFLPs
• Place into electrophoresis chamber
• Separation based on size and charge
• Stain gel
• Analyze using a light box

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DNA Fingerprinting
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DNA Fingerprinting
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DNA Fingerprinting
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DNA Fingerprinting