NCCS is an autonomous institution

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NCCS is an autonomous institution of the
department of Biotechnology, Govt. of India It
serves following functions. National Repository
for animal cells, obligate parasites, plasmids
and vectors Human Resource Development in the
fields of animal tissue culture Research and
Development in the frontier areas of biomedical
sciences
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Cell Repository NCCS is the only tissue culture
facility of its kind present in India catering
the need of researchers dependent on usage of
human/animal Cells. The achievements of cell
repository have grown from 100 odd cell lines to
over a thousand from a broad spectrum of species.

• Tissue Banking
• Indigenously developed a microcapsule generator
• assembly for islet encapsulation.
• Encapsulated islets in chitosan-alginate
  microcapsules
• for transplantation and Islet banking

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Technology Transfer
Lab grown skin technology Technology has been
developed and transferred to Hospitals in Pune,
Mumbai and Delhi. It is an important achievement
for the faster healing of burned wounds. Bone
marrow and cord blood cryopreservation The
center has developed improvised technologies for
crypreservation and revival of human bone marrow
and cord blood cells. The technical know-how has
been transferred to Armed Forces Medical college,
Pune. One successful transplant of bone marrow
has been achieved.
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• Tissue Engineering
• An interdisciplinary branch that applies the
  principles of engineering
• and life sciences towards development of
  biological substitutes that
• restore maintain or improve tissue function
• Need for Tissue Engineering
• High cost involved in treating patients with
  organ failure
• Shortage of donar tissues available for
  transplantation
• Failure of synthetic prosthesis to replace all
  the functions of lost
• organ and incapability adapting to bodys
  changing needs

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• Materials in Tissue Engineering
• Biocompatibility essentially involves
• Non-cytotoxicity
• Hostocompatibility
• Hemocompatibility
• Immunocompatibility
• Non-mutagenicity
• Materials used can be biodegradable e.g. PLA, PGA
  and Chitosan.
• Biodegradable materials degrade slowly inside the
  body to form
• monomers which are non-toxic and are metabolized.

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Design and Development of Biomaterials for Drug
Delivery

• pH dependent controlled release system for
  gastric
• delivery of amoxicillin
• Freeze-dried chitosan-PVP semi-IPN hydrogel
• High porosity and specific area
• pH dependent swelling and controlled delivery
• over period of 3 h hence ideal for gastric
  delivery
• Controlled release system for intestinal delivery
• Freeze-dried chitosan-polyacrylamide hydrogel
• Controlled delivery of amoxicillin over period of
  75 h

Risbud et al. J. Control Release 68 (1) 23-30,
2000, Risbud et al. Drug Delivery 7(2), 69-75,
2000
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Biohybrid Pancreas development

• Crosslinked hydrogels and natural polymeric
  membranes
• Suitable physico chemical properties
• Mechanically stable
• Selective molecular weight cut off preventing
  passage of insulin Ab
• Permit diffusion of insulin, glucose and
  micronutrients
• Immunocompatible with macrophages and lymphocytes
  
• Compatible with endothelium
• Maintain morphology, viability and functionality
  of islets

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Risbud et al. Cell Transplant 9(1) 25-30, 2000,
Cell Transplant 10(2)195-202, 2001 Risbud et al.
J. Biomed. Mater. Res 54(3), 436-444, 2001,
Risbud et al. J. Biomed. Mater. Res (In press)
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