Microbial Detection Arrays

1
Microbial Detection Arrays
MiDAs
Shayla Stewart Steven To Charles Vaughan Sameera
Wijesinghe
Dave Miller Elizabeth Newton Ted Schumacher
Interim Review I February 6th, 2007 Aerospace
Senior Projects University of Colorado
Boulder Advisors Dr. Forbes and Dr.
Maslanik Customers BioServe and Tufts University
2
Briefing Overview

• Overall Design Summary
• Changes Since CDR
• Test Plans
• Schedule
• Budget
• Current Issues

3
Overall Design Summary
Reagent Water Chamber
Autoclaves
Peristaltic Pump Mounts
Top Shelf
Environmental Chamber
Reaction Chambers (with Mixers)
NOTE Only shows parts to be manufactured
Mixer Motor Mounts
4
Changes Since CDR

• Mechanical
• Autoclave Repositioning of screw holes and
  O-ring groove
• Mixer Rearrangement of impellers, addition of
  bottom scoop
• Environmental chamber Walls broken up to
  simplify manufacturing and assembly
• Shelves and supports Sizes standardized, COTS
  supports
• Electrical
• Sensors LM34 temperature sensors on autoclave,
  reagent water chamber, and ambient
• PP-0079 thermistors on reaction chamber
• Additional thermistors placed on reaction
  chambers
• Software
• Laptop hard drive for data storage
• Manufacturing
• BioServe manufacturing parts of autoclave bodies
  (milling), reaction chamber bodies, and mixers

5
Changes Since CDR
Electrical System
Mixer
Rectangular Mixing Blades
Four Controlled Items
Flat Scoop
6
Test Plan Flow Chart
Power Supply Circuit
Reagent H2O Containment
Software Input/Output Voltage
Switch Board Circuit
Sample Transport
Integrated Electrical Subsystem
Integrated System Verification
Peristaltic Pump Control Circuit
Mixer Control Circuit
Mixing Capability
Disassembly/ Reassembly
Sample Sterility
Autoclave Heater
Autoclave Temp/Pressure
Autoclave Kill Test
TEC Control Circuit
Rxn Chamber Environ. Control
7
Special Test Requirements

• Hazard Analyses
• Electrical electrocution/fire
• Autoclave pressure vessel (projectiles), hot
  surfaces, sharp edges
• Assembly/Disassembly sharp edges
• Environmental control hot surfaces
• Integrated System all of above
• Testing Locations
• BioServe Environmental Chamber
• BioServe Wet Chemistry Lab

8
Schedule
9
Budget
Expected Actual
Electronics 0 0 (donated)
Sensors 0 0 (donated)
Mechanical 1,328.53 1,909.61
Computer/DAQ 348.00 606.00
Miscellaneous 150.00 328.01
Total 1,826.53 2,843.62
10
Current Issues

• Tubing
• PharMed tubing does not come in 1 inner diameter
• Substitution has been found (Bioprene) but has
  not been ordered
• TEC Controller
• Power cord did not come with TEC controller
• Replacement can be fabricated or purchased
• Manufacturing
• Two weeks behind schedule
• Offset by manufacturing contributions from
  BioServe
• Ordering
• Pumps, thermostats, and TECs have not been ordered

11
Conclusions

• Manufacturing behind schedule
• Offset by BioServes assistance
• Most parts are ordered
• Tubing is only ordering concern
• Can be offset by finding other distributors
• Still on budget
• Over what was expected, but still under budget
  with most parts ordered
• Mechanical, software, and electrical packages
  approved
• Will be able to complete manufacturing and
  testing by deadlines