mohankandaswamy

1
Oriental Aquamarine Biotech India Private
LimitedCoimbatore
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Nitrifying Bioreactor Technology

• Biological Filtration System
• Live activated bacterial consortium
• For Removal of Ammonia, Nitrite and Nitrate
• Research program initiated by Govt. of India in
  1995
• Cumulative result of 16 years of R D
• Technology developed and validated at NCAAH,
  CUSAT
• Technology licensed to Oriental Aquamarine
  Biotech.

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Nitrifying Bacterial Consortium
Ntspa 712 Nitrospira
Cysts in AMONPCU
NIT2 Nitrobacter sps
ß ammonia oxidizer
Nitrosomonas AMONPCU
NmV Nitrococcus mobilis
linage
DAPI
EUBB 338 Bacteria
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Molecular Characterization of Bacterial
Consortium
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Nitrifying Bacterial Consortium

• Nitrifying bacterial consortium contains
  different beneficial bacteria
• Ammonia oxidizing bacteria
• Nitrosomonas, Nitrosococcus, and Nitrosospira
• Nitrite oxidizing bacteria
• Nitrobacter, Nitrococcus, and Nitrospira
• Aerobic de-nitrifiers
• Pseudomonas denitrificans and Paracoccus
  denitrificans
• Perform de-nitrification by using NO3 as electron
  acceptor in place of oxygen

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Media

• Low Density Poly Ethylene beads of 5 mm diameter
  with active surface area 0.785 sq.cm
• Bacterial Consortium grow as biofilm on beads

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Stringed Bed Suspended Bioreactor (SBSBR)
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Packed Bed Bioreactor (PBBR)
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System Hydraulics

• Max flow rate - 50 Lpm
• Capacity 60000 litres /day
• Bioreactor design is modular.
• Can be customized as per requirements
• Design of Reactor completed for 1 million litres
  per day

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Progress Of Nitrification In Aquaculture System
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Comparison
OAM Others
Specific bacterial consortium    
Onsite activation 2 3 days 6 8 weeks
Nitrification and de-nitrification One reactor Two reactors
Nitrate removal  
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• IP Status
• Patented in India
• Patent No 241648
• PCT
• International Publication No-WO02/30835A1
• International patent classification C02f 3/13
• International application No. PCT/IN00/00097
• Patented in South East Asian countries
• Thailand
• Japan
• Philippines
• Indonesia and
• South Korea.

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Waste Water Treatment

• System currently operational in Aquaculture
  sector.
• Has potential for tertiary treatment of domestic
  and industrial waste water.
• Lab Scale Experiments completed.

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Lab Scale Experiments

• Samples collected after primary and secondary
  treatments
• Ammonia load 30ppm
• Trial with Activated Stringed Bed Suspended
  Bioreactor (SBSBR).
• 30 ppm TAN converted within 24 hours
• 1 ppm reduction of Ammonia, results in reduction
  in 7 ppm of Alkalinity (carbonate/bicarbonate)
• Addition of Base required to balance pH and
  maintain at 7.8

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Domestic Sewage Waste Water
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Metal Industry Waste Water
Treatment
1st tube-NH3 2nd tube-NO2 3rd tube-NO3
1st tube-NH3 2nd tube-NO2 3rd tube-NO3
1st tube-NH3 2nd tube-NO2 3rd tube-NO3
1st tube-NH3 2nd tube-NO2 3rd tube-NO3
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Results

• NH3 reduced to 0 ppm within 24 hours.
• Presence of heavy metals like Cadmium, Nickel,
  Lead, Copper does not affect the nitrification
  efficiency of the bioreactor

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Trials with 150 ppm Ammonia loading
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Treatment with 150 PPM of Ammonia

•  Gradual Increase in Reactor efficiency
• pH monitored and optimized to 8.5
• Initial Removal Rate 60 ppm per day
• Current Removal Rate 80 ppm - 100 ppm per day.

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Optimum condition for Operation  
S.NO Particulars Minimum Maximum
1 Temperature 27 C 30 C
2 pH 7.5 8.5
3 Alkalinity 100 200
4 Dissolved oxygen 3 PPM 6 PPM
5 Salinity 0 PPT 5PPT
6 Turbidity 0 PPM 50 PPM
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Nitrifying Bioreactor Technology

• System operational in lab scale.
• Process can be scaled up to treat high volume of
  effluent.
• Can be used for Tertiary Treatment of Water for
  removal of Nitrogenous Compounds

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• Thank you
• Mohan Kandaswamy,
• Oriental Aquamarine Biotech India Pvt. Ltd
• U-7, Kovaipudur
• Coimbatore-42
• www.nitrifying-bioreactor.com
• 0422-2608943