Histopathology Laboratory

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How to Choose the Best Stain for your Histology
Slides?
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Histopathology Laboratory

• Using stains in microscopy is a routine part of
  the daily operations in a histopathology
  laboratory. The purpose of using these stains is
  to enhance contrast and highlight the structures
  in the slides, and thus to get accurate results
  in the tests.
• However, choosing the best stain for accurate
  results isnt always easy. This is because there
  are numerous stains in histology and selecting
  the best is cumbersome.

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Here we give you a glimpse of the commonly used,
and some specialized stains, their purpose, and
utility to guide you in selecting the right stain
for your histology slides.
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The Most Commonly Used Stains
Haematoxylin and eosin are the first and most
common stains used in most histopathology labs.
Both these stains are used for routine tissue
study where you dont need much detail.

• Haematoxylin stains all the nucleic acids (like
  DNA and RNA), and eosin stains the basic
  structures like muscle or red blood cells.
• Haematoxylin stains the cell nuclei as bluish
  purple while eosin dyes other cell structures
  pink or red.

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Specific Stains for your Histology Slides
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1) Gram Stain

• Stain the slide with crystal violet and let it
  stand for a minute.
• Gently rinse the slide with tap water or
  distilled water from a wash bottle and smear it
  with Grams iodine and potassium iodide solution.
  Let it stand for one minute.
• Gently rinse the slide with tap water or
  distilled water from a wash bottle and
  decolourise with alcohol and acetone.
• Follow this by counter-staining with a red or
  pink stain and let it stand for 45 seconds.

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2) Congo Red

• A stain which was once used in textile industry
  has now become popular in the histopathology
  laboratory for detecting amyloid fibres. These
  fibres get stained as a pale orange-red colour
  with the Congo stain.
• Detection of amyloid fibres is important for the
  diagnosis of diseases like Alzheimers,
  Parkinsons, and Diabetes.
• With routine stains like haematoxylin and eosin,
  its not possible to detect bacteria, amyloid
  fibres and other details in the study tissue.
  Thus, these specific stains must be a part of
  your histopathology laboratory.

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• If you dont have accesses to these stains or get
  stuck with any process in the lab, feel
  comfortable to outsource your work to a
  knowledgeable lab.
• Exploit the histology services of a reputed lab
  to save money, time, and get high-quality slides
  with accurate results. Competent histology
  services have an experienced lab team to assist
  you in your daily microscopy operations and make
  your investments worthy.

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Professional Histology Services
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To Know More

• Contact Us
• Email ID project_at_cognibrain.com
• Phone [phone]
• Visit us https//www.cognibrain.com/