Histopathology Laboratory Services

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How to Prepare High-Quality Histopathology
Slides?
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Before you can analyze a specimen in a lab

• You must prepare a perfect histology slide of the
  specimen

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Preparing a high-quality histological slide is
not easy!
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Follow this step-by-step guide to prepare one!
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Step 1

• Fix the tissue sample
• After collection, transfer the tissue into a
  container of 10 neutral buffered formalin
• Cap the container tightly and keep so for 24-48
  hours
• Ensure the container is not leaking and label it
  correctly

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Step 2

• Transfer the tissue to histology cassette
• Trim the tissue specimen using a scalpel
• Make it appropriate to fit into the cassette
• Keep the edges of the specimen thin, less than
  4mm
• Store the cassette in formalin

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Step 3

• Process the tissue specimen
• Immerse the specimen in increasing level of
  alcohol to remove any water or formalin from it
• Clean the specimen with xylene
• Embed the specimen in paraffin wax
• Ensure the wax surrounds the tissue and forms a
  block

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Step 4

• Section the tissue specimen
• Remove wax from tissue surface
• Keep the tissue block in a refrigerator for 10
  minutes
• Slice the specimen into thin ribbons using a
  microtome
• Transfer the tissue slices to a warm water bath

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Step 4 (contd.)

• Place a charged slide under the water level to
  scoop the tissue slices on the slide surface
• Label the slides correctly
• Allow them to dry in upright position at 37C

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Step 5

• Stain the slides
• Use a histochemical stain like hematoxylin or
  eosin to stain the tissue specimen on the slide
• Attach a cover slip after staining for long-term
  protection of the slide

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Preparing a high-quality slide is not only
complex, but also time consuming
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How many slides you need to prepare in a day?

• Countless

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Do you think you can prepare so many slides
without a mistake?
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Do you have a trained staff for preparing slides?
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Is your answer No?
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Then Outsource Your HistologyServices
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To Whom?
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A Histopathology Laboratory that offers
cost-effective histology services
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What do histology services offer?

• Processing of tissues
• Preparation of slides
• Specific histochemical analysis
• Immunohistochemistry
• Immunoflourescence

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To Know More

• Email ID project_at_cognibrain.com
• Phone [phone]
• Visit us https//www.cognibrain.com/
• Connect with us