Title: Yeast Expression Systems in Creative BioMart
1Yeast Expression Systems in Creative BioMart
Creative
BioMart
2As a well-defined and economical eukaryotic
expression system for both secretory and
intracellular expression, yeast protein
expression system is perfectly suitable for
large-scale manufacture of recombinant proteins.
Creative BioMart has developed yeast expression
vectors that are optimized for the efficient
production of heterologous proteins. We have also
a few strong signal sequences to ensure the
highest secretion level.
3High yield High productivity Genetics can be
easily manipulated Chemically defined
media Complex post-translational
modifications Stable production
strains Durability Ease of scaling up Low protein
production cost
4At Creative BioMart, our expertise in protein
expression extends to both Saccharomyces.
cerevisiae and Pichia. pastoris expression
systems. Expression of recombinant protein in S.
cerevisiae can be done using three types of
vectors
5Yeast integrating plasmid Replicating single copy
plasmid For integration into genomic locus
6Yeast centromeric plasmid Replicating single copy
plasmid For complementation studies
7Yeast episomal plasmid Replicating multi-copy
plasmid For recombinant protein production,
multicopy suppression studies, etc.
8Saccharomyces. cerevisiae
Full genomic sequence known Well developed
genetic methods About 6000 genes A model to other
eukaryotes GRAS No toxins Ability to secrete
proteins Capacity for post-translational
modifications
9Besides, methylotrophic Pichia.pastoris is a
distinguished system for its growth to very high
cell densities, for the available strong and
tightly regulated promoters, and for the options
to produce gram amounts of recombinant protein
per liter of culture. If you're looking for
protein production via fed-batch pichia
processes, we have the experienced and
credentialed staff to move your project forward.
10Thanks for watching! Contact Creative
BioMart Address 45-1 Ramsey Road Shirley, NY
11967, USA Email contact_at_creative-biomart.com W
ebsite http//www.creativebiomart.net