Protein Crystallization

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Protein Crystallization
by Creative Biostructure
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Creative Biostructure offers high-throughput
UniCrys crystallization services with our
state-of-the-art facilities and has developed an
X-ray crystallography pipeline including all the
stages from gene synthesis to structure
determination. Our experienced scientists can
teamwork with customers and accelerate the
research progress.
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X-ray crystallography is a scientific technique
of determining the positions and arrangements of
atoms in a crystal. Beams of X-ray diffract into
various specific directions when they strike a
crystal, forming a diffraction pattern that can
be interpreted into the three-dimensional
structure of macromolecules. Since the first
determined protein structure myoglobin in
1958, X-ray crystallography has become the most
significant and useful tool for protein structure
determination. Nowadays, more than 115,000
protein structures have been deposited into the
Protein Data Bank and about 90 of them are by
X-ray, suggesting its overwhelming advantages in
protein structure study.
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The key step in X-ray crystallography is the
crystallization process. Since each protein has
its unique physical and chemical properties, the
initial crystallization screening can be very
costly and time consuming. However, by taking
advantages of our in-house X-ray diffraction
platform, Creative Biostructure can provide the
most efficient and economical strategies. The
detailed services we provide are summarized in
Table 1.
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Service
Co-crystallization
Co-crystallization retains the unique
crystalline structures with their multiple
components (e.g., proteins, DNA/RNA, chemical
compounds, metal ions). Peptide Crystallization
Great for crystallization of small peptides,
especially flexible peptides without predefined
conformation. Membrane Protein Crystallization
Crystallization of membrane proteins while
maintaining the native conformations and
retaining their functions and activities.
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The structural biology team at Creative
Biostructure carries out high-resolution
structure determination by using both in-house
X-ray facility (for trial and optimization) and
synchrotron light source (for data collection).
High-resolution X-ray data, high-quality density
maps, protein structures and statistics will also
be made available for the purpose of publication
and subsequent studies.
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Contact Creative Biostructure Mailing Address
45-1 Ramsey Road Shirley, NY 11967, USA Email
Address contact_at_creative-biostructure.com Websi
te http//www.creative-biostructure.com/protein-c
rystallization_26.htm