Chromatography

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Chromatography

• Fighting Crime in the Lab.

2
A much loved pet (and incidentally a very
valuable breed), has been DOGNAPPED!
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Our client is on the left. The dog is on the
right.
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The ransom Note
Put 10000 in a plastic bag. I will tell you
where to go. Do not tell the police or Fido gets
it.
The note appears to have been written with a
marker pen.
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We shall analyse the ink used in the ransom note.
It might help to identify the type of pen used.
The ransom note has been processed to recover
some of the ink. The chromatography cell will
separate out the dyes used in the ink.
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Just to be sure what we want

• Read the notes for today, know what they say,
  glue them in.
• DO the standard procedure, ie the chromatography
  experiment.
• Write a summary in your book, explaining how the
  process works.
• GAS Chromatography look at this last theres a
  simple fact sheet.

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A small (VERY SMALL) sample is squirted into the
system and blown through a column by an inert gas.
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The sample is vapourised but condenses onto the
walls of the column. The components creep along
the column depending on their attractions to the
column lining or to the gas.
Various sorts of Detectors can be used. This one
works by measuring the amount of charge that each
generates when ionised in a hydrogen flame. This
is complicated. Lets discuss it with me and
each other.
9
Look at the fact sheet. Draw your own version of
the diagram ie, a Block Diagram, of the Gas
chromatograph. Label it. For extra brownie
points, describe how it works.