Protein Synthesis: A Simulation of Transcription and Translation

1
Protein Synthesis A Simulation of Transcription
and Translation
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Promotor Region
Terminator Sequence
Coding Strand
TATA Box
5 AATATATTGCTCCGATGTTTGCTGAAAAACGATTTCGCTGACCGAA
TAA 3
3 TTATATAACGAGGCTACAAACGACTTTTTGCTAAAGCGACTGGCTT
ATT5
5AUAUUGCUCCGAUGUUUGCUGAAAAACGAUUUCGCUGACCGAAUAA3
Template Strand
RNA polymerase begins to transcribe the template
strand near the end of the promotor region.
and stops at the terminator sequence ..AATAA3
..TTATT5
TRANSCRIPTION
mRNA
3
Promotor Region
Terminator Sequence
Coding Strand
TATA Box
5 AATATATTGCTCCGATGTTTGCTGAAAAACGATTTCGCTGACCGAA
TAA 3
3 TTATATAACGAGGCTACAAACGACTTTTTGCTAAAGCGACTGGCTT
ATT 5
Template Strand
RNA polymerase begins to transcribe the template
strand near the end of the promotor region.
and stops at the terminator sequence ..AATAA3
..TTATT5
TRANSCRIPTION
Post-transcriptional modification of the primary
transcript occurs in the nucleus.
1 addition of 5 cap of methyl guanosine
2. addition of 3 poly-adenosine tail
mRNA
AUAUUGCUCCGAUGUUUGCUGAAAAACGAUUUCGCUGACCGAAUAA
5metG
AAAAA3
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Promotor Region
Terminator Sequence
Coding Strand
TATA Box
5 AATATATTGCTCCGATGTTTGCTGAAAAACGATTTCGCTGACCGAA
TAA 3
3 TTATATAACGAGGCTACAAACGACTTTTTGCTAAAGCGACTGGCTT
ATT 5
Template Strand
RNA polymerase begins to transcribe the template
strand near the end of the promotor region.
and stops at the terminator sequence ..AATAA3
..TTATT5
TRANSCRIPTION
Post-transcriptional modification of the primary
transcript occurs in the nucleus.
1 addition of 5 cap of methyl guanosine
2. addition of 3 poly-adenosine tail
mRNA
AUAUUGCUCCGAUGUUUGCUGAAAAACGAUUUCGCUGACCGAAUAA
5metG
AAAAA3
1. mRNA is translated in the 5 to 3 direction
2. Translation begins with the start codon
(AUG)
TRANSLATION

• Translation ceases with one of three stop
  codons, (UGA, UAG or UAA)

met
- phe
- ala
- glu
- lys
- arg
- arg
- phe
methionine - phenylalanine - alanine -glutamic
acid - lysine - arginine - phenylalanine -
arginine
Polypeptide
5
Promotor Region
Terminator Sequence
Coding Strand
TATA Box
5 AATATATTGCTCCGATGTTTGCTGAAAAACGATTTCGCTGACCGAA
TAA 3
3 TTATATAACGAGGCTACAAACGACTTTTTGCTAAAGCGACTGGCTT
ATT 5
Template Strand
RNA polymerase begins to transcribe the template
strand near the end of the promotor region.
and stops at the terminator sequence ..AATAA3
..TTATT5
TRANSCRIPTION
Post-transcriptional modification of the primary
transcript occurs in the nucleus.
1 addition of 5 cap of methyl guanosine
2. addition of 3 poly-adenosine tail
mRNA
AUAUUGCUCCGAUGUUUGCUGAAAAACGAUUUCGCUGACCGAAUAA
5metG
AAAAA3
1. mRNA is translated in the 5 to 3 direction
2. Translation begins with the start codon
(AUG)
TRANSLATION

• Translation ceases with one of three stop
  codons, (UGA, UAG or UAA)

methionine - phenylalanine - alanine -glutamic
acid - lysine - arginine - phenylalanine -
arginine
Polypeptide
6
Phenylalanine tRNA
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Protein Synthesis Animations
http//bcs.whfreeman.com/thelifewire/content/chp12
/1202001.html
http//207.207.4.198/pub/flash/26/transmenu_s.swf
http//highered.mcgraw-hill.com/sites/0072437316/s
tudent_view0/chapter15/animations.html
Polyribosome Animation
http//www.sumanasinc.com/webcontent/animations/bi
ology.html
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Textbook References
Nelson Biology 12 5.2 Protein Synthesis An
Overview Section 5.2 Questions 1 11, p
241 5.3 Transcription Section 5.3 Questions 1
9, p 249 5.4 Translation Section 5.4
Questions 1 9, p 254
Campbell Biology Ch 17 From Gene to Protein
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