CELL FRACTIONATION

1
(No Transcript)
2
CELL FRACTIONATION

• Divide by MASS

3
(No Transcript)
4
ELECTRON MICROSCOPES

• Better magnification and resolution due to
  shorter wavelengths of electrons! .

5
Limits of Microscopy
6
Electron Microscope
7
Scanning Electron Microscope (SEM)

• A beam of electrons is focused across the
  surface of an object.
• Benefit Super 3-dimensional images of the
  surface of an object.
• Magnification 300,000 Xs

8
Transmission Electron Microscope (TEM)

• Focuses a beam of electrons through an image.
• Benefit Great images and detail within a cell.
• Magnification up to 500,000 Xs

9
Any drawbacks?

• Specimen must be in a vacuum.
• Specimens dehydrated and preserved.
• Specimens must be very thin, less than 0.1
  micrometers for the TEM.
• No living organisms in an electron microscope.
• Also, they are very expensive and require
  extensive training.

10
TEM vs SEM
11
(No Transcript)
12
Cross-section of one cilia or flagellum
13
What is it?What type of scope would deliver
this type of image?