Barcoding Marine Fishes: A ThreeOcean Perspective

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Barcoding Marine Fishes A Three-Ocean Perspective
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Barcoding All Marine Fishes 15 000 Species
Barcoding All Marine Fishes
Target 15 000 species
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Recovering Barcodes from Fishes
Taxonomic Coverage 530 Species (3.5) 317
Genera (10.5) 130 Families (32) 28 Orders
(55)
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Distribution Map
Atlantic
Pacific
Indian
Collection Site
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Within Region Analysis
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Within Region South Africa (254 species)
Conspecific
X 0.61 0.12
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Within Region Portugal (45 species)
Conspecific
X 0.29 0.04
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Within Region Canada (52 species)
Conspecific
X 0.48 0.08
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COI Divergences ()
98 Species Possessed Unique Barcodes
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Potentially Overlooked Diversity (Within Region)
Conspecific
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Apparent Sequence Sharing
Region Sequence Sharing Canada
0.84 South Africa 1.08 Australia
0.14 Portugal - Overall
0.40

• Explanations
• Problematic Taxonomy
• Error
• Laboratory
• Misidentification
• Identical Barcodes
• Introgressive hybrization
• Recently diverged taxa

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Apparent Sequence Sharing
Congeneric
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Morphologically Problematic Taxa - Sebastes
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Specimen Vouchering

• Whole Specimen
• South Africa
• Portugal
• Tissue and Image
• Canada
• Tissue Only
• Australia

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Between Region Analysis
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Potentially Overlooked Diversity (Between Regions)
South Africa vs. Australia

• 25 Species
• 6 Pelagic
• 19 Reef/Inshore

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Offshore Pelagics
Range 0 0.62
Mean 0.20 0.03
Reef Associated
Range 1.95 16
Mean 7.56 0.43
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Conclusions

• Highly conserved priming regions
• High species resolution within each region
• Species discovery through DNA barcodes
• Specimen vouchering

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Collaborators
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Acknowledgments
Acknowledgements
Laboratory
Database
Sujeevan Ratnasingham
Jeremy deWaard
Rob Dooh
Nataly Ivanova
Pia Marquardt
Janet Topan
Funding Support
Angela Holliss
Gordon and Betty Moore Foundation
Collections
Jim Boutillier
NSERC CFI OIT
Allan Connell
Canada Research Chairs Program
Peter Last
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