Nematode Extraction Methods: Foliar Extraction Techniques

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Nematode Extraction MethodsFoliar Extraction
Techniques

• Kavitha Govindasamy
• Jimmy R.Rich
• Maria L .Mendes

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A Plant-Parasitic Nematode
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Sampling for Nematodes

• Sampling and extracting for nematodes serves two
  purposes
• Diagnosis a current problem
• Predict a future problem

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Objectives of sampling and extraction

• Identify the nematode
• Population estimation
• Diagnose a nematode disease
• Make management decisions
• Regulatory purposes
• Research and surveys

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Some Common Nematode Extraction Methods

• Foliar samples
• Punching and incubating
• Scissors technique
• Soil samples
• Baermann funnel
• Modified Baermann funnel
• Root Samples
• Jar incubation and sieving
• Blending and sieving

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Foliar Extraction TechniquesUsing a punch or
scissors

• Principle
• Nematodes migrate out of the leaves when the
  
• lesions are soaked in water

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Materials Required
Hole punch or scissors Petri dish or other
container
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Cut leaf tissue and place in water
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Punch the lesions and place tissue disks in water
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Take samples near healthy tissue
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Incubate 24 hours and observe under microscope
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Acknowledgments

• Janete Brito, Department of Plant Industry,
  Gainesville, Florida
• Frank Woods, Nematode Assay Lab, University of
  Florida, Gainesville, Florida
• Joey Orajay, Entomology and Nematology
  Department, University of Florida, Gainesville,
  FL

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For additional information or comments, please
contact

• Kavitha_nem_at_yahoo.com
• jimmyr_at_ufl.edu
• mlmendes_at_ufl.edu