Title: Introduction to Genomics
1 2Post genomics ?Knowledge of the function of a
genome
- What is genomics ?
- Knowledge of the whole genome of an organism
3- Why sequence a genome ?
- How do you sequence a genome ?
4- Many organisms have now been sequenced
5(No Transcript)
6- A new sequencing project
- How would you start ?
Rapid lifecycle Apomyctic Commercial
interest Transgenics ? Genome size ? Linkage
groups ? Funding ?
Slow lifecycle Can be cloned Commercial
interest Transgenics ? Genome size ? Linkage
groups ? Funding ?
7- Practical step 1.
- Do you have a model framework to fix your
genome on ? - Synteny
8Crop circles
Gale Devos
9Cat vs human synteny
Why cats ?
10Human/Mouse Synteny
-The order of genes within eachblock of DNA is
conserved in different species even though the
blocks have assembled to give vastly different
numbers length of chromosomes
11- - orientation of genes are conserved too
- - Gene pairs that are syntenic are most likely
orthologous (direct evolutionary counterpart
Related by vertical descent)
12Human/mouse synteny (gene level)
- Gene structures such as regulatory regions,
splice sites, exon number, exon length and
sequence similarity (DNA and protein level) tend
to be more conserved - Intronic sequences and
length tend to be less conserved
13- Step 2.
- Map your genome
- Genetic maps
- Physical maps
14Arabidopsis recombinant inbred maps micro-linkag
e groups Made from the performance or markers
across 2-300 lines
15- Step 3.
- Break your genome up into manageable fragments
- YAC
- BAC
- Cosmids
- ESTs (expressed sequence tags)
16STS (sequence tagged site) Short (200 to 500 bp)
DNA sequence that has a single occurrence in the
genome and whose location is known.
17Minimal tiling path
BAC tiling path - mouse
18- Sequence the bitsOld fashioned. -OR-
next-gen e.g. Solexa or 454 - Stick on ESTs to show regions of genes
- Analyse by example (training sets) to develop
in-silico predictions of unknown genes.
19- Global analysis
- What does the genome look like ?
20What do the genes do ?