Title: F plasmid integrates into chromosome by recombination
1Donor chromosome
F cell
1
F plasmid integrates into chromosome by
recombination
Hfr cell
2Donor chromosome
F cell
1
F plasmid integrates into chromosome by
recombination
Hfr cell
Pilus
2
Cells join via a conjugation pilus
F cell
F recipient
3Donor chromosome
F cell
1
F plasmid integrates into chromosome by
recombination
Hfr cell
Pilus
2
Cells join via a conjugation pilus
F cell
F recipient
Copy of donor DNA
F plasmid
Part of F plasmid
3
F plasmid partially replicates and moves into
recipient cell trailing a copy of donors DNA
4Donor chromosome
F cell
1
F plasmid integrates into chromosome by
recombination
Hfr cell
Pilus
2
Cells join via a conjugation pilus
F cell
F recipient
Copy of donor DNA
F plasmid
Part of F plasmid
3
F plasmid partially replicates and moves into
recipient cell trailing a copy of donors DNA
Incomplete F plasmid cell remains F
4
Conjugation ends with pieces of F plasmid and
donor DNA in recipient cell
F cell
Hfr cell
5Donor chromosome
F cell
1
F plasmid integrates into chromosome by
recombination
Hfr cell
Pilus
2
Cells join via a conjugation pilus
F cell
F recipient
Copy of donor DNA
F plasmid
Part of F plasmid
3
F plasmid partially replicates and moves into
recipient cell trailing a copy of donors DNA
Incomplete F plasmid cell remains F
4
Conjugation ends with pieces of F plasmid and
donor DNA in recipient cell
F cell
Hfr cell
5
Donor DNA and recipient DNA recombine making a
recombinant F cell
Recombinant cell