Genetic Mapping ? ?? ??

1
Genetic Mapping ? ?? ??
A ???? B ??? ??? ??(genetic map ???? Double
heterozygote x Double homozygote AaBb
aabb
2
?? ???? ??? ??? ?? ??? ???

• 1) ??? mapping? ??? ????? ??? ????? ??? ??? ? ??.
  
• 2) ????? ??? ??? ???? ??? ???? ???? ??? ??? ???,
  ???? ????? ? ? ? ???? ??? ???.

3

• ??? ??? mapping? ?? 3??
• ??? ?? ???
• ?conjugation
•  ?transduction
•  ?transformation

4
Restriction mapping

• ???? ????? ??? ???? ??
• RFLP marker? ?? mapping ? ??? ? ?? (?? marker
  ?? ??)
• ???? ??? ??? ??? ??(?? ??(50 Kb ??)? ??? ??)
• 50 Kb ??? ?? seven or eight-nucleotide? ????
  ???? ??

5
?? ??????? ??

• OFAGE
• CHEF
• Optical Mapping- ?????
• -gel stretching
• -molecular combing

6
FISH

• ????? ??? probe? ??
• In situ Hybridization on chromosome
• Metaphase chromosome
• ? ??? FISH
• -Mechanically stretched chromosome(????)
• -Non-metaphase chromosome(interphase)

7
STS mapping

• High resolution-FISH ?? ??
• STS sequence tagged site
• -EST
• -SSLP
• -random genomic sequence
• STS mapping reagent ? ??

8
STS mapping reagent

• Radiation hybrid
• ?? ??? ??? ??
• Hamster ??? ??
• Clone library
• High capacity vector ???? ?? insert ? ?? Kb ???
  cloning ?
• ???? ???? library? ?? ? ??

9
Physical mapping

• ??? ??? ???? ???? mapping
• 1. Restriction mapping
• 2. FISH
• 3. STS mapping