Molecular Microbiology

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Molecular Microbiology
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Genetic Recombination

• Vertical
• Binary fission
• Budding
• Horizontal
• Conjugation
• Transduction
• Transformation

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Conjugation
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Transduction
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Transformation
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Griffith Experiment
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Biotechnology

• Recombinant DNA
• Genetic Engineering
• Disease treatments
• SCID
• SSA
• CF
• Make products
• Vaccines
• Enzymes
• Insulin
• Hormones

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Plasmids

• DNA
• Replicate independently
• Genetic Vectors
• Small
• Stable
• Marker inserted
• Gene expression

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Background

• Donor
• Recipient
• Competent Cell
• Natural
• Change cell wall
• Change PM
• Artificial
• Temperature
• Salts (CaCl2)
• Marker
• Cloning Site

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LB Broth and Agar

• Lysogeny Broth
• Luria Broth
• Luria-Bertani
• Nutritionally Rich
• Peptides, casein
• Vitamins
• Minerals (NaCl)
• Trace elements
• Biotech
• Cultivate E.coli

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Procedure

• Follow lab manual!!
• Reader
• Directs
• Keeps time
• Materials
• LB plates
• 2 types
• LB
• LB-amp
• 2 situations
• Before
• After
• Label, Label, Label
• pGreen plasmid
• CaCl2
• Micropipets
• Spreader
• Loop inoculation
• Temperature

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Results

• Marker
• Fluorescence
• Cloning site
• R-ampicillin
• Colony observation
• Natural light
• UV light
• Calculate transformation efficiency

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Questions?
Glow Fish