4ta Reunin Annual PulseNet Amrica Latina

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4ta Reunión Annual PulseNet América Latina
Buenos Aires, Argentina
June , 2006
Experience of Instituto Adolfo Lutz, Central
Public Health Laboratory, São Paulo State, Brazil
, on the use of standardized Pulsed-Field Gel
Electrophoresis - PulseNet Protocol
Tânia Mara Ibelli Vaz
Instituto Adolfo Lutz São Paulo, Brazil
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SÃO PAULO STATE
BRASIL












Instituto Adolfo Lutz

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Government of São Paulo State
Secretary of Health, São Paulo
Coordination of Diseases Control
CCD
Center of Sanitary Surveillance
Center of Epidemiological Surveillance
Section of Bacteriology
Instituto Adolfo Lutz
Division of Medical Biology
Dept. Micro Immunol
Enterobacteria and other Gram negative
Mycobacteria
Meningo, Pneumo, Haemophilus,Enteroc
Leptospira
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Instituto Adolfo Lutz
Central Public Health
Laboratory São Paulo
State, Brazil
v National Reference Laboratory for Escherichia
coli (NRLEC)
Salmonella
Shigella Vibrio cholerae
v Regional Reference Laboratory (RRL)

Strains to NRLEC / RRL sent by
National Public Health
Laboratories NRLEC Regional Laboratories
of Instituto Adolfo Lutz (11 Laboratories)
Private and Public Hospital
Laboratories

Paraná



Santa Catarina

RRL Central Public Health
Laboratories Rio Grande do Sul

Mato Grosso do Sul
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Characterization of Salmonella Enteritidis
strains isolated from foodborne outbreaks and
from foods associated with these outbreaks
Fig 1B. XbaI PFGE patterns of S.Enteritidis
strains
Fig.1A. XbaI PFGE patterns of S.Enteritidis
strains
1 2 3 4 5 6 7 8 9 10
1 2 3 4 5 6 7 8 9 10
1,5,10 , molecular marker, S. Braenderup H9812
2,3,6,7,8 and 9 , S. Enteritidis strains from
humans 4, S. Enteritids from mayonnaise
1,5,10 molecular marker S. Braenderup H9812
4,6,7,8 and 9, S.Enteritidis strains from
humans 2 and 3, S.Enteritidis from food
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Characterization of Salmonella Johannesburg
strains isolated from humans and foods
Fig. 2. XbaI PGFE patterns of S. Johannesburg
strains from humans and from foods
1 2 3 4 5 6 7
8 9 10
1,5,10 , molecular marker, Salmonella Braenderup
H9812 2, 3 and 4, S. Johannesburg strains
from humans 6,7,8 and 9, S. Johannesburg
strains from foods
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Salmonella Typhi strains isolated from two
typhoid fever (TF) outbreaks and from sporadic
cases of TF
Fig.3. XbaI PFGE patterns of SalmonellaTyphi
1 2 3 4 5 6
7 8
1,8 , molecular, marker, S.Braenderup H9812
2,3, S.Typhi from sporadic cases of TF 4,5
STyphi from outbreak A 6,7, S.Typhi from
outbreak B
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Characterization of Salmonella I 1,4,5,12i-
and S.Typhimurium strains isolated from human
and non- human sources (1991-2000)
Fig 4A. XbaI PFGE patterns of Salmonella I
1,4,5,12i-
Fig 4B. XbaI PFGE patterns of SalmonellaTyphimur
ium
1 2 3 4 5 6 7 8 9 10 11 12
13 14 15
1 2 3 4 5 6 7 8 9 10 11 1 13
14 15

1,8,15 Molecular marker, S. Braenderup H9812 (
XbaI)2,3,4,5,6,7,9,10,11,12,13,14 , Salmonella
I1,4,5,12i-
1,8,15 molecular marker S. Braenderup H9812
2,3,4,5,6,7,9,10,11,12,13,14 S. Typhimurium
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Characterization of Salmonella Typhimurium
isolated from sporadic cases of human diseases
and from non-human sources (2003-2004)
Fig.5 XbaI PFGE patterns of SalmonellaTyphimurium
1 2 3 4 5 6 7 8 9
10 11 12 13
1,7,13 molecular marker, S. Braenderup H9812
2,3,4,5,6,8,9,10,11,12 S. Typhimurium strains
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Characterization of E.coli O157H7
Fig 6.XbaI PFGE patterns of E.coli O157H7
1 2 3 4 5 6
7 8 9 10
1,5 and10- molecular marker 2,3,4,6,7,8 and 9
E.coli o157H7 strains
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Tânia Mara Ibelli Vaz - VazTMI Instituto Adolfo
Lutz Av.Dr.Arnaldo 351, 9o andar CEP
01246-902 Cerqueira Cesar São Paulo SP Brazil
taibelli_at_ial.sp.gov.br vaztmi_at_uol.com.br