RECIPE

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RECIPE
Charquemont October 23-27th 2003
Ecobio Rennes
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Contents
Guillaume
Nathalie
ECOBIO team
André-Jean

• Carbon analyzer
• Nitrogen analyzer
• WP1 Eriophorum angustifolium site
• Regeneration in Baupte
• Trenches in Baupte
• WP1 Rotifer sampling
• Questions

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C apparatus Bioritech Carbon analyzer

• Characteristics and principle
• chemical oxydation coupled with low thermic
  action (gt 90
• and lt 100 C)
• Direct estimations of TIC and TOC in the sample
• Calibration, standardization

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N apparatus Büchi analyzer

• Characteristics and principle
• distillation with NaOH and measuring out with
  H2SO4 (Kjeldahl method)
• - measuring out N forms, inorganic and organic
  with Kjeldahl method (except NO3) or Dewarda
  methods (NO3 and NH4)

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WP1-Eriophorum angustifolium site

• Setting the experimental design in Baupte
• E. angustifolium recolonization since 1999
  (regeneration beginning lt 10 yrs)
• Moving the cylinders in (see yellow posts)

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Regeneration of turfigenous process
? (g C m-2 an-1) Production - Losses
-70 à 27 soit A/P 0 à 0,14
-264 à - 80 soit A/P 0
(A accumulation, P Production)
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WP2 3 - Trenches in Baupte

• The water table is very low this year

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WP2 3 - Trenches in Baupte

• Digging 3 ditches to ajust the position of the
  trench next time (after natural rewetting with
  rain).

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WP1 - Rotifer sampling

• Rotifers are numerous in green and dead parts of
  Sphagnum
• Hypothesis they influence the microbial loop
  functioning
• Difficulty species need to be determined on
  living individus
• Sampling material
• What partners should do demonstration in the
  field
• Collected samples need to be send in cold
  containers (about 5C, not exceed 10 C)
• Analyses in Ecobio
• Pressing
• Filtration
• Rotifer determination

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Questions- Adjustments

1. PLFA Complementary work with Münich
   microbial and microfauna communities
2. Fate of C and N litter in the peat column (WP3)
   choice of the compartments to be taken into
   account for 15N and 13C analyses
3. Peepers and chemical elements to be analysed (see
   Philips table and AJs precisions)

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The end thank you very much