Microscopic World

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Microscopic World
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By Daniella Galipo, Robert Peluso and Tamsyn
Connolly
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• Lesson overview
• Observing and learning the parts of the
  microscope
• How to use the microscope
• How to make a wet slide
• The importance of staining
• Differences between animal and plant cells

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The Microscope
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Example Worksheet Labelling the parts of the
microscope
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Letter e Experiment

• Objectives
• Students will learn the function of each part of
  a microscope.
• Students will observe that the letter "e" is
  upside down when viewed under low power.
• Students will notice when the slide with the
  letter "e" is moved in one direction, it appears
  to move in the opposite direction.
• Students will observe how the letter "e" looks
  when viewed from low to medium and to high power.

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Introducing cell types

• Differences between animal and plant cells
• e.g. Cell wall, cell membrane, different
  organelles, etc.
• Onions used to view plant cells
• Cheek cells used to examine animal cells

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Wet mount slide
Onion cells

• Remove a thin piece of onion skin tissue from the
  onion
• Place the tissue on a slide
• Add a drop of iodine stain
• Carefully place the cover slip on top of the
  onion cells
• Observe under low, medium and high magnification
• Record observations

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Wet mount slide
Cheek cells

• Scrape inside of cheek with a cotton swab
• Place the tissue on a slide
• Add a drop of methylene blue stain
• Carefully place the cover slip on top of the
  cheek cells
• Observe under low, medium and high magnification
• Record observations

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Staining

• Staining is required to view the cells under
  the microscope
• Onion cell Iodine stain
• Reacts with starch to stain cells brown, making
  nuclei more visible
• Cheek cell Methylene blue stain
• Stains cells blue, making nuclei more visible

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10x magnification
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40x magnification
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Comparison of cells
Onion Cells
Cheek Cell
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Summary

• Microscopes are effective tools in teaching
• Can be used in the teaching of cells and
  micro-organisms
• Enables students to be actively involved in
  their own learning

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THE END