The giant squid axon preparation

1
The giant squid axon preparation
2
(No Transcript)
3
(No Transcript)
4
(No Transcript)
5
(No Transcript)
6
(No Transcript)
7
(No Transcript)
8
(No Transcript)
9
(No Transcript)
10
(No Transcript)
11
From channels to action potentials
12
(No Transcript)
13
(No Transcript)
14
Patch pipette methods giga-ohm seal for
excellent voltage-clamp
15
(No Transcript)
16
From individual channel kinetics to summed
population current
17
Inactivation of the fast Na channel
18
Voltage-sensitive open probability in a K
channel yields mM curve
Channel open/closed times reflect open/closed
two-state rate constants
19
Diverse types of firing behavior mediated by a
host of interacting K and Ca currents
Anomalous rectifier
Low-threshold burst
Intrinsic bursting
Spike accommodation
20
Excitable dendrites
21
Calcium channels inactivated by intracellular
Ca
22
The low-threshold T-type Calcium current
(thalamo-cortical neuron)
20 mV
Vm rest
Iinj 0
0.5 nA
50 ms
23
Firing modes in neurons with low-threshold
calcium currents (thalamic)
Steady spike-mode
Burst-mode
24
Neuromodulation of firing mode behavior
thalamo-cortical states
ACh or NE
50 ms
Burst-mode (sleep)
Transmission-mode (awake)
Reticular thalamic neuron (inhibitory)
Thalamo-cortical neuron
25
(No Transcript)
26
(No Transcript)
27
(No Transcript)
28
(No Transcript)