Gene Structure

1
Gene Structure
2
Recombinant DNA, Cloning, Restriction Enzymes
3
Cloning DNA Fragments
Lodish, Figure 7-3
4
Polymerase Chain Reaction (PCR)
5
Link to PCR movie
6
Southern Blotting
7
Arabidopsis
small genome size (100 Mbp) fast
regeneration time (6-8 weeks) requires small
growth space easily transformed easy to
characterize/map mutations
8
Similarity Between Arabidopsis and Rice Genes
9
Functional Classification of Predicted Genes in a
Region
10
Functional Comparison Between Organisms
11
Rice
12
Agrobacterium Transformation
13
Electroporation
14
Biolistic (Gene) Gun
15
Mutagenesis
16
T-DNA Insertion
17
Maize and Transposons
18
Maize Transposition
19
Maize Transposition
20
Reporter Gene in Arabidopsis
K.A. Johnson
21
LacZ Expression in a Mouse
This demonstrates The expression of a Reporter
gene in mice.
22
GUS Staining in Gene Trap Line
23
ProliferaGFP in Dividing Root Cells